Flag f3165 antibody

A3G (60100-1) and Rab7 (55469-1-AP) antibodies were purchased from Proteintech. Antibodies against Alix (A2215), TSG101 (A2216), and VDAC1 (A0810) were purchased from ABclonal. Peroxidase-labeled β-actin antibody (A3854) and Flag antibody (F3165) were purchased from Sigma. nSMase2 (sc-166637) antibody was purchased from Santa Cruz Biotechnology. HA antibody (M20003) and eGFP antibody (M20004) were purchased from Abmart. Mouse monoclonal HBx antibody was custom made as previously described43 (link).

HDAC1 (ab7028), HDAC2 (ab7029), HDAC3 (ab32369), HDAC8 (ab18968), histone H3 (ab70550), and HIV-1 Tat (ab43014) antibodies were purchased from Abcam. VprBP (A301-888A) antibody was from Bethyl Laboratories. Flag antibody (F3165) was from Sigma-Aldrich. GAPDH (14C10) and rabbit IgG isotype control (2729) were from Cell Signaling Technology. Anti-p24-PE (sc-69728 PE) was from Santa Cruz. Ace H3K9 (39917) and Ace H4K5 (39699) antibodies were from active motif. Mouse and rabbit HRP-conjugated antibodies were from Abcam. Monoclonal antibody against a conserved region of HIV-1 Vpr was raised in mouse by conjugating KLH (keyhole limpet hemocyanin) to a synthetic peptide (EAVRHFPRHWLHGLGQ) corresponding to amino acids 29–44 of Vpr prevalent among clinical isolates. SYBR Select Master Mix was from Life Technologies. Benzonase nuclease was from Novagen. Caffeine, Flag peptides, SAHA, and DMSO were from Sigma-Aldrich. MG132 was from Millipore. VprBP and non-targeting siRNAs were from Dharmacon. HDAC1 siRNA was from Qiagen. HDAC3 siRNA was from Santa Cruz Biotechnology. HDAC-Glo™ I/II Reagent was from Promega.

BAP1 (#13271S), CBX3 (#2619S), BRD4 (#13440S), CDK9 (#2316S), H3K27Ac (#8173S), H2AK119Ub (#8240), NSD3 (#92056S), JMJD6 (#60602S), CHD4 (#12011S), CCNT1 (#81464S), Pol II (#14958), histone H3 (#4499S), H3K4me1 (#5326S), and BRD2 (#5848S) antibodies were purchased from Cell Signaling. HSP90 (sc-7947) and GFP (sc-9996) antibodies were purchased from Santa Cruz. HCFC1 (A301-399A), BRD3 (A302-368A), and FOXK1 (A301-728A) antibodies were purchased from Bethyl Laboratories. Tubulin antibody (E7) was purchased from Developmental Studies Hybridoma Bank. Flag (F3165) antibody was purchased from Sigma. ASXL3 antibodies were made in-house with antigen peptides against ASXL3 (AA 1250-1399) and ASXL3 (AA 1405-1699). BAP1 ChIP-seq antibody was generated as described previously [4 (link)]. JQ1 (1268524-70-4) was purchased from MedChemExpress. Both dBET6 (S8762) and IBET-151 (S2780) were purchased from Selleckchem.