Hcm hepatocyte culture medium bulletkit

Manufactured by Lonza

Sourced in Singapore

The HCM Hepatocyte Culture Medium BulletKit is a complete, serum-free medium designed for the culture of primary human hepatocytes. It provides the necessary components to support the growth and maintenance of these cells in vitro.

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Lab products found in correlation

Hepg2, by National Collection of Authenticated Cell Cultures (1 mentions) Bel 7404, by National Collection of Authenticated Cell Cultures (1 mentions) Hucs10p, by Lonza (1 mentions) Qgy 7701, by American Type Culture Collection (1 mentions) Sk hep1, by National Collection of Authenticated Cell Cultures (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions) Lx 2 cells, by Merck Group (1 mentions) L glutamine, by Sartorius (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Mycoprobe mycoplasma detection kit, by R&D Systems (1 mentions) Hepg2 c3a cells, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

HCM Hepatocyte Culture Medium Hepatocyte culture medium HCM medium BulletKits

3 protocols using hcm hepatocyte culture medium bulletkit

Hepatocyte Cell Lines and Knockout

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The LO-2 human normal hepatic cell line and the QGY-7701 and HUH7 HCC cell lines were obtained from ATCC. The HepG2, Bel-7404, SK-Hep-1, and Hep3B HCC cell lines were obtained from the National Collection of Authenticated Cell Cultures (NCAC) in China. Primary human hepatocytes (HH, mixed donor) were obtained from Lonza (HUCS10P). Cell lines were cultured with Eagle's minimum essential medium (EMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco). HH cells were cultured in HCM™ Hepatocyte Culture Medium BulletKit™ (CC-3198, Lonza). All cells were incubated at 37°C and 5% CO₂. hsa-miR-582-5p knockout (miRKO) cell lines were generated by GenePharma with the CRISPR/Cas9 system.

Ren Y., Lyu J., Guo Y., Yao Y, & Hu L. (2022). Long Noncoding RNA TUG1 Inhibits Tumor Progression through Regulating Siglec-15-Related Anti-Immune Activity in Hepatocellular Carcinoma. Journal of Immunology Research, 2022, 9557859.

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Establishing Primary Dermal Fibroblast Cultures

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Primary dermal fibroblast cultures were established from skin biopsies from patients F1-II:1 and F2-II:2 and two unaffected and unrelated individuals (WT1 and WT2), following standard procedures⁷⁷. Primary dermal fibroblast cell lines, HEK293T cells (ATCC, catalog no. CRL-3216, RRID:CVCL_0063) and LX-2 cells (Millipore, catalog no. SCC064, RRID: CVCL_5792, gift from H. Weiping (Duke-NUS Medical School, Singapore)) were cultured in complete High Glucose Dulbecco’s Modified Eagle Medium (HyClone) supplemented with 10% fetal bovine serum (FBS) (Biological Industries) and 2 mM L-glutamine (Biological Industries). Hc3716-hTERT cells (a gift from P. Kaldis (Institute of Molecular and Cell Biology, A*STAR, Singapore)) were cultured in HCM Hepatocyte Culture Medium BulletKit (Lonza, catalog no. CC-3198) supplemented with 5% FBS and 10% human serum (Sigma-Aldrich, catalog no. H4522). All cell lines were maintained in a humidified atmosphere at 5% CO₂ and 37 °C and tested negative for mycoplasm using the MycoAlert Mycoplasma Detection Kit (Lonza, catalog no. LT07-118).

Traspas R.M., Teoh T.S., Wong P.M., Maier M., Chia C.Y., Lay K., Ali N.A., Larson A., Mutairi F.A., Al-Sannaa N.A., Faqeih E.A., Alfadhel M., Cheema H.A., Dupont J., Bézieau S., Isidor B., Yanwen Low D., Wang Y., Tan G., Lai P.S., Piloquet H., Joubert M., Kayserili H., Kripps K.A., Nahas S.A., Wartchow E.P., Warren M., Bhavani G.S., Dasouki M., Sandoval R., Carvalho E., Ramos L., Porta G., Wu B., Lashkari H.P., AlSaleem B., BaAbbad R.M., Abreu Ferrão A.N., Karageorgou V., Ordonez-Herrera N., Khan S., Bauer P., Cogne B., Bertoli-Avella A.M., Vincent M., Girisha K.M, & Reversade B. (2022). Loss of FOCAD, operating via the SKI messenger RNA surveillance pathway, causes a pediatric syndrome with liver cirrhosis. Nature genetics, 54(8), 1214-1226.

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Cell Culture Maintenance Protocol

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HepG2/C3A cells were obtained from the American Type Culture Collection (ATCC, CRL-10741) and maintained in Eagle's Minimum Essential Medium (ATCC) with 10% FBS (HyClone). HepaRG 5F parental cells were purchased from Sigma-Aldrich and maintained with the HCM Hepatocyte Culture Medium BulletKit (Lonza). Cells were incubated in a humidified atmosphere at 37°C with 5% CO₂ and routinely verified to be mycoplasma free by using the MycoProbe Mycoplasma Detection Kit (R&D Systems). Cell counts were obtained with a Countess II Automated Cell Counter using trypan blue staining.

Huber A.D., Poudel S., Wu J., Miller D.J., Lin W., Yang L., Bwayi M.N., Rimmer M.A., Gee R.R., Seetharaman J., Chai S.C, & Chen T. (2023). A bromodomain-independent mechanism of gene regulation by the BET inhibitor JQ1: direct activation of nuclear receptor PXR. Nucleic Acids Research, 52(4), 1661-1676.

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