Low igg fbs

Vero.E6 cells (ref) were seeded in 96 well plates (Corning; 3340) at a seeding density of 2x10⁴ cells per well 24 hours prior to infection with NDV-HexaPro-S^{82 (link)} at a multiplicity of infection (MOI) of 2. One hour later the inoculum was removed and replaced with 100μl of Roswell Park Memorial Institute (RPMI-1640) media (Thermofisher). 48 hours later the media was removed and replaced with 25 μL of RMPI-1640 media supplemented with 2% low-IgG FBS (Promega). Antibodies were serially diluted 1:10 in RPMI 1640 media from a starting concentration of 30 μg/mL to a final concentration of 0.014 μg/mL and 25 μL of antibody dilution was added to the plate. CR9114 was utilized as a negative control^{48 (link)}. MAb dilutions were not added to 16 wells per plate to provide background luminescence readings. 3x10⁶ ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO₂ for 6 hours. 75 μL of Bio-Glo luciferase reagent (Promega) was then added to each well and the luminescence was read in a Synergy 4 (BioTek) plate reader. The background luminescence reading was used to calculate AUC using GraphPad Prism 10.