Liver tissues were collected by surgical manipulation, and fixed using 10% buffered formalin solution (Histo-Fresh, Pharma, Tokyo, Japan). Total lipids were extracted using a mixture of methyl tert-butyl ether (Wako Pure Chemical, Osaka, Japan) and methanol (Wako Pure Chemical, Osaka, Japan), as previously reported [37 (link)]. The dried lipid residues were dissolved in 20 µL of isopropanol containing 10% Triton X-100 (Nakarai Tesque, Kyoto, Japan). TG and TCHO were measured using Wako L-type TG and Wako L-type TCHO (Wako Pure Chemicals, Tokyo, Japan) assay kits according to the manufacturer’s protocol.
Methyl tert butyl ether (mtbe)
Manufactured by Fujifilm
Sourced in Japan
Methyl tert-butyl ether is a colorless, volatile liquid used as a chemical reagent and solvent in various laboratory applications. It serves as a key component in analytical techniques and sample preparation processes.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Fujifilm (2 mentions)
Triton x 100, by Nacalai Tesque (1 mentions)
Wako l type tg, by Fujifilm (1 mentions)
Wako l type tcho, by Fujifilm (1 mentions)
Trifluoroacetic acid, by Fujifilm (1 mentions)
Pyrogallol, by Fujifilm (1 mentions)
Acetonitrile, by Fujifilm (1 mentions)
Fluorescein, by Nacalai Tesque (1 mentions)
Folin ciocalteu reagent, by Nacalai Tesque (1 mentions)
Methyl β cyclodextrin, by Fujifilm (1 mentions)
2 2 azobis 2 amidinopropane dihydrochloride, by Fujifilm (1 mentions)
β carotene, by Fujifilm (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchromane 2 carboxylic acid, by Merck Group (1 mentions)
β cryptoxanthin, by Funakoshi (1 mentions)
Heptane, by Fujifilm (1 mentions)
Ethyl acetate, by Fujifilm (1 mentions)
Trifluoroacetic acid tfa, by Fujifilm (1 mentions)
9 aminoacridine 9 aa, by Merck Group (1 mentions)
Isopropanol ipa, by Fujifilm (1 mentions)
Fisher optima grade, by Thermo Fisher Scientific (1 mentions)
4 protocols using methyl tert butyl ether (mtbe)
1
Lipid Extraction and Quantification
2
Antioxidant Evaluation and Characterization
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The following reagents were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan): 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), acetonitrile, methanol, methyl tert-butyl ether (MTBE), methyl-β-cyclodextrin, β-carotene, trifluoroacetic acid (TFA), and pyrogallol. Fluorescein and Folin-Ciocalteu reagent were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Additionally, 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA), and β-cryptoxanthin was purchased from Funakoshi Co., Ltd. (Tokyo, Japan).
3
Comprehensive LC-MS Lipid Analysis
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Ultrapure water (Fisher Optima grade, Fisher Scientific, Pittsburgh, PA) was used for liquid chromatography (LC)-MS. HPLC-grade chloroform (CHCl 3 ), methanol (MeOH), methyl-tert-butyl ether (MTBE), butanol, heptane, ethyl acetate, acetonitrile (ACN), hexane, isopropanol (IPA), and trifluoroacetic acid (TFA) were purchased from Fujifilm Wako Pure Chemical Corp. (Osaka, Japan). Proteomics-grade hydrochloric acid (HCl) was also obtained from Fujifilm Wako Pure Chemical Corp. 9-Aminoacridine (9-AA) and 8 M molecular biologygrade lithium chloride (LiCl) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2,5-Dihydroxybenzoic acid (DHB) was obtained from LaserBio Labs (Sophia Antipolis Cedex, France). All lipid internal standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA) and are listed in (17:0/20:4) ]; phosphatidylglycerol [PG (17:0/20:4)]; and phosphatidylinositol [PI (21:0/22:6) ]. PC (14:0/14:0) and PG (17:0/14:1) were obtained from Avanti Polar Lipids, Inc. and used as external ionization standards (Table 1-2).
4
Carotenoid Extraction and Analysis
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2.1 Chemicals Lutein, zeaxanthin, and β-cryptoxanthin were purchased from Extrasynthese (Genay, France) . α-Carotene and methyl tert-butyl ether (MTBE) were obtained from Wako Pure Chemical Industries (Osaka, Japan) . β-Carotene and lycopene were obtained from Sigma-Aldrich (St. Louis, MO, USA) . All other reagents were of the highest grade available.
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