Thb agar tha

The bacterial strains and plasmids used in this study are listed in Table 1, strain SC19 was isolated from a diseased pig during an epidemic outbreak in 2005 in Sichuan, China (Li et al., 2009 (link)). The mnmE deletion mutant of SC19 (ΔmnmE) obtained from our previous research were also used in this study (Gao et al., 2019 (link)). The SS2 were grown in Todd-Hewitt broth (THB; Oxoid, Basingstoke, England) or on THB agar (THA; Oxoid, Basingstoke, England) plates supplemented with 5% sheep blood (Maojie, Nanjing, China) at 37°C. The arginine metabolic pathway study was performed using a chemically defined medium (van de Rijn and Kessler, 1980 (link)). Escherichia coli (E. coli) strain DH5α (Vazyme, Nanjing, China) used as host strain for cloning, were grown in LB broth (Difco Laboratories, Franklin Lakes, NJ, USA) or on LB agar plates at 37°C. If necessary, erythromycin (90 μg/ml), spectinomycin (100 μg/ml), and streptomycin (20 μg/ml) were supplemented to promote bacterial selection.

The bacterial strains and plasmids used in this study are listed in Table 1. The virulent SS2 strain SC19 was isolated from a diseased pig during the 2005 epidemic outbreak in Sichuan of China (Li et al., 2009 (link)). The S. suis strains were grown in Todd-Hewitt broth (THB; Oxoid, Basingstoke, England) or on THB agar (THA; Oxoid, Basingstoke, England) plates supplemented with 5% sheep blood (Maojie, Nanjing, China) at 37°C. The arginine metabolic pathway study was performed using a chemically defined medium (van de Rijn and Kessler, 1980 (link)). Erythromycin (90 μg/mL) was added to screen for the mutant strain, while erythromycin (90 μg/mL), and spectinomycin (100 μg/mL) were added to select for the complemented strain. The E. coli DH5α strains were grown in LB broth (Difco Laboratories, Franklin Lakes, NJ, USA) or on LB agar plates at 37°C. If necessary, kanamycin (25 μg/mL) was added.