Mhc gs gal4

Wild-type, dSesnXP4 (UAS-dSesn) and dSesn^8A11 (dSesn^−/−) were previously produced in Exelixis w¹¹¹⁸ background. Mef2-Gal4 (#27390), MHC-GS-Gal4 (#43641), ap-Gal4 (#3041), UAS-dAKT (#8191), UAS-dAKT^RNAi (#33615), UAS-dSin1^RNAi (#32371), UAS-4E-BP^RNAi (#36667), UAS-dTSC2^RNAi (#34737), UAS-dPGC1^RNAi (#33915), UAS-dSnf1^RNAi (#32371) and fly lines with an attP landing platform were obtained from the Bloomington Drosophila Stock Center (BDSC, Bloomington, IN, USA). UAS-dSesn^RNAi-A (#38481) and UAS-dSesn^RNAi-B (#104365) were obtained from the Vienna Drosophila RNAi Center (VDRC, Vienna, Austria). UAS-dPGC1 is a kind gift from David Walker. dSesn cDNA14 of wild-type (dSesn^WT) and C86S(dSesn^CS), D424A (dSesn^DA) or D423A/D424A (dSesn^DDAA) mutated forms were previously produced and are described in Kim et al., [30 (link)]. C86, D423 and D424 in dSesn correspond to C125, D406 and D407 in mammalian SESN2.
Flies were grown and aged on 10% sugar-yeast medium at 25 °C, 50% humidity, and 12/12 h light/dark cycle. Control flies for all non-gene-switch UAS Gal4 experiments consisted of both the UAS and Gal4 lines into Attp or w¹¹¹⁸, as appropriate. For experiments in which both the Gal4 control and UAS control flies were found to be statistically identical, results were pooled and graphed as a single datum. In gene-switch experiments, RU-flies of the same genotype served as the negative control.

Fly stocks used in this study were Act5c-GS-Gal4, WB-FB-GS-Gal4 (a combination of S32-GS-Gal4 and S106-GS-Gal4), and MHC-GS-Gal4, which were described in a previous study.^{6 (link)} The elav-GS-Gal4 (#43642) and UAS-mCherry (#35787) were obtained from the Bloomington Drosophila Stock Center. The wild-type strains used were B3 and w¹¹¹⁸. UAS-foxo fly stocks were also used.^{7 (link)}