Silencer® Select pre-designed siRNA oligonucleotides targeting IFITM3 were purchased from Ambion and tested: siRNA ID #s195033, #s195034, and #s195035 (sense sequence 5΄-CCCACGUACUCCAACUUCCtt −3΄ and antisense sequence 5΄-GGAAGUUGGAGUACGUGGGat-3΄). IFITM3 siRNA (#s195035) and scrambled siRNA (negative control) were used. siRNAs were transfected to HEK293-APP695 cells in triplicates as described previously50 . The conditioned medium was measured for secreted Aβ40 and 42 levels by using Aβ peptide multiplex kits (6E10, MSD) according to manufacturer’s instructions. To check cell viability, Alamar Blue (AbD Serotec) was added to the cells and incubated50 . Transferred medium in a 96-well black polystyrene microplate (Costar) was read for fluorescence (530–560 nm excitation/ 590 nm emission) by EnVision Plate Reader (PerkinElmer). Protein knock-down levels were measured in cell lysates by WB. siRNA transfection in HEK293-NotchΔE cells was performed as described above. Levels of protein knock-down, NotchΔE and NICD were measured in cell lysates by WB.
Silencer select pre designed sirna oligonucleotides
Manufactured by Thermo Fisher Scientific
Silencer® Select pre-designed siRNA oligonucleotides are synthetic short interfering RNA (siRNA) molecules designed to target and silence specific genes. These oligonucleotides are pre-designed and optimized to provide effective gene knockdown while minimizing off-target effects.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using silencer select pre designed sirna oligonucleotides
1
Silencing IFITM3 Impacts Amyloid-beta Secretion
2
Silencing IFITM3 Impacts Amyloid-beta Secretion
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Silencer® Select pre-designed siRNA oligonucleotides targeting IFITM3 were purchased from Ambion and tested: siRNA ID #s195033, #s195034, and #s195035 (sense sequence 5΄-CCCACGUACUCCAACUUCCtt −3΄ and antisense sequence 5΄-GGAAGUUGGAGUACGUGGGat-3΄). IFITM3 siRNA (#s195035) and scrambled siRNA (negative control) were used. siRNAs were transfected to HEK293-APP695 cells in triplicates as described previously50 . The conditioned medium was measured for secreted Aβ40 and 42 levels by using Aβ peptide multiplex kits (6E10, MSD) according to manufacturer’s instructions. To check cell viability, Alamar Blue (AbD Serotec) was added to the cells and incubated50 . Transferred medium in a 96-well black polystyrene microplate (Costar) was read for fluorescence (530–560 nm excitation/ 590 nm emission) by EnVision Plate Reader (PerkinElmer). Protein knock-down levels were measured in cell lysates by WB. siRNA transfection in HEK293-NotchΔE cells was performed as described above. Levels of protein knock-down, NotchΔE and NICD were measured in cell lysates by WB.
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