The following commercial primary antibodies were: anti-14-3-3 (1657, SantaCruz, Dallas, TX, USA); anti-Actin (MAB1501, Chemicon International-Fischer Scientific, Waltham, MA, USA); anti-CaMKIIα (M1-048, ThermoFisher Scientific, Waltham, MA, USA); anti-Caveolin (ab2910, Abcam, Cambridge, UK); anti-Clathrin (610500, BD Biosciences, Franklin Lakes, NJ, USA), anti-Flotillin (610820, BD Biosciences, Franklin Lakes, NJ, USA), anti-MAP2 (M9942 clone HM-2, Sigma-Aldrich, Sant Louis, MO, USA); anti-NCAM2 (AF778, R&D Systems, Minneapolis, MN, USA)); anti-NCAM2.1 (EB06991, Everest, Oxfordshire, UK); anti-NF200 (N4142, Sigma-Aldrich, Sant Louis, MO, USA) and anti-Nogo (11027, Santa Cruz, Dallas, TX, USA).
Anti flotillin
Manufactured by BD
Sourced in United States
Anti-flotillin is a lab equipment product that functions as an antibody. It is used for the detection and analysis of flotillin proteins in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti caveolin, by Abcam (1 mentions)
Anti 14 3 3, by Santa Cruz Biotechnology (1 mentions)
Anti nf200, by Merck Group (1 mentions)
Anti clathrin, by BD (1 mentions)
Ecl system, by GE Healthcare (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Hrp linked secondary antibody, by GE Healthcare (1 mentions)
Anti alix, by Santa Cruz Biotechnology (1 mentions)
Anti calnexin, by Stressgen (1 mentions)
Anti p stat3, by Cell Signaling Technology (1 mentions)
Supersignal chemiluminescence ecl kit, by Merck Group (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Anti erk1 2 antibody, by Cell Signaling Technology (1 mentions)
Anti β tubulin, by Merck Group (1 mentions)
Fusion fx5 imaging system, by Vilber (1 mentions)
Anti stat3, by Santa Cruz Biotechnology (1 mentions)
Anti p erk1 2, by Cell Signaling Technology (1 mentions)
3 protocols using anti flotillin
1
Antibody Panel for Neuronal Analysis
2
Western Blot Analysis of EV Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were recovered by centrifugation and pellets were resuspended in lysis buffer (62 mM Tris–HCl pH 6.8, 11% glycerol, 1% SDS containing phosphatase and protease inhibitors) and sonicated to prepare cell lysates.
Aliquots of cell lysates (15 μg proteins) or EVs (2 μg proteins) were mixed with sample buffer 5X (1 M Tris–HCl pH 6.8, 5% (w/v) SDS, 6% (v/v) glycerol, 0.01% (w/v) Bromophenol blue). Samples were electrophoresed on 12% acrylamide gel and electrotransferred to PVDF membrane. After blocking, membranes were incubated overnight with the following primary antibodies: anti-Alix (Santa Cruz, USA), anti-CD63 (Cymbus Biotechnology, UK), anti-β-actin (Sigma-Aldrich, USA), (Santa Cruz, USA), anti-calnexin (Stressgen, USA), anti-flotillin (BD Biosciences, Franklin Lakes, USA), anti-Apo B (EMD Millipore Corp, USA). HRP-linked secondary antibodies (GE Biosciences, Piscataway, USA) were probed according to manufacturer’s instructions. Immunoblots were detected by chemiluminescence using ECL system (GE Biosciences).
Aliquots of cell lysates (15 μg proteins) or EVs (2 μg proteins) were mixed with sample buffer 5X (1 M Tris–HCl pH 6.8, 5% (w/v) SDS, 6% (v/v) glycerol, 0.01% (w/v) Bromophenol blue). Samples were electrophoresed on 12% acrylamide gel and electrotransferred to PVDF membrane. After blocking, membranes were incubated overnight with the following primary antibodies: anti-Alix (Santa Cruz, USA), anti-CD63 (Cymbus Biotechnology, UK), anti-β-actin (Sigma-Aldrich, USA), (Santa Cruz, USA), anti-calnexin (Stressgen, USA), anti-flotillin (BD Biosciences, Franklin Lakes, USA), anti-Apo B (EMD Millipore Corp, USA). HRP-linked secondary antibodies (GE Biosciences, Piscataway, USA) were probed according to manufacturer’s instructions. Immunoblots were detected by chemiluminescence using ECL system (GE Biosciences).
3
Immunoblotting Analysis of Cytokine Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were mixed with 2× Laemmli buffer prior denaturation at 95°C for 5 min. Samples were then subjected to SDS-PAGE and immunoblotting. Reagents for electrophoresis were purchased from BioRad and polyvinylidene fluoride membranes were from Millipore. The following antibodies (1:1000 dilution) were used: rabbit monoclonal anti-CNTFRα, anti-LIFRβ and anti-gp130 antibodies (Santa Cruz Biotechnology), anti-P-STAT3 (STAT3 phosphorylated on Tyr705) and anti-P-ERK1/2 (ERK1/2 phosphorylated on Tyr202/Thy204) antibodies (Cell Signaling Technology), mouse monoclonal anti-β-tubulin (Sigma), anti-flotillin (BD Biosciences), anti-STAT3 (Santa Cruz Biotechnology) and anti-ERK1/2 antibodies (Cell Signaling Technology). Goat anti-rabbit IgG and horse anti-mouse IgG peroxidase-conjugated secondary antibodies (Cell Signaling Technology) were detected using Supersignal chemiluminescence (ECL kit; Millipore). Approximate molecular weights were estimated using PageRuler prestained Protein Ladder Plus (Euromedex). Densitometric analysis of immunoblot images was performed using the Fusion Fx5 Imaging System (Vilber Lourmat) and ImageJ software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!