The protein lysates from treated cells were run on an 8% SDS-PAGE gel and transferred onto a PVDF membrane. Then, the membrane was blocked with 5% fat-free milk overnight at 4 °C. The next day, the membrane was incubated with the primary antibodies (MET(D-4)/sc-514148, p-MET(F-5)/sc-377548, STAT3(F-2)/sc-8019, p-STAT3(B-7)/sc-8059, Santa Cruz, USA; AKT(pan) (C67E7)/#4691, p-AKT/#406, ERK(1/2) (137F5)/#4695, p-ERK(Thr202/Tyr204) (D13.14.4E)/#4370, WTAP/#5650, METTL3 (D2I6O)/#96391, METTL14(D8K8W)/#51104, EGFR/#2232, pEGFR(Y1068)/#2234, Cell Signaling Technology, USA; FTO/ab126605, Abcam, UK; and GAPDH/60004-1, Protein Tech, China) at room temperature for at least 1 hr. Then, the membrane was washed with PBST (1×PBS with 0.1% Tween 20) three times at an interval of 10 min. After washing, the membrane was incubated with the appropriate goat anti-rabbit (SE131, Solarbio, China) or goat anti-mouse (SE131, Solarbio, China) secondary antibodies at room temperature for 1 hr. After washing 6 times, the signals were visualized using the Immobilon Western Chemiluminescent HRP Substrate Kit (WBKLS0500, Millipore, Billerica, USA).
Stat3 f 2 sc 8019
Manufactured by Santa Cruz Biotechnology
Sourced in United States
STAT3(F-2)/sc-8019 is a primary antibody that targets the STAT3 (Signal Transducer and Activator of Transcription 3) protein. It is a mouse monoclonal antibody specific for an epitope mapping between amino acids 685-770 of STAT3 of human origin.
Automatically generated - may contain errors
Lab products found in correlation
Immobilon western chemiluminescent hrp substrate kit, by Merck Group (2 mentions)
Gapdh 60004 1, by Proteintech (2 mentions)
Pegfr y1068, by Cell Signaling Technology (2 mentions)
Met d 4 sc 514148, by Santa Cruz Biotechnology (2 mentions)
P met f 5 sc 377548, by Santa Cruz Biotechnology (2 mentions)
P stat3 b 7 sc 8059, by Santa Cruz Biotechnology (2 mentions)
P akt 406, by Cell Signaling Technology (2 mentions)
Mettl3 d2i6o, by Cell Signaling Technology (2 mentions)
Mettl14 d8k8w, by Cell Signaling Technology (2 mentions)
Fto ab126605, by Abcam (2 mentions)
Egfr 2232, by Cell Signaling Technology (2 mentions)
2 protocols using stat3 f 2 sc 8019
1
Western Blot Analysis of Signaling Proteins
2
Western Blot Analysis of Cell Signaling
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The protein lysates from treated cells were run on an 8%-12% SDS-PAGE gel and transferred onto a PVDF membrane. Then, the membrane was blocked with 5% fat-free milk overnight at 4 °C. The next day, the membrane was incubated with the primary antibodies (MET(D-4)/sc-514148, p-MET(F-5)/sc-377548, STAT3(F-2)/sc-8019, p-STAT3(B-7)/sc-8059, Santa Cruz, USA; AKT(pan) (C67E7)/#4691, p-AKT/#406, ERK(1/2) (137F5)/#4695, p-ERK(Thr202/Tyr204) (D13.14.4E)/#4370, WTAP/#5650, METTL3 (D2I6O)/#96391, METTL14(D8K8W)/#51104, EGFR/#2232, pEGFR(Y1068)/#2234, Cell Signaling Technology, USA; FTO/ab126605, Abcam, UK; and GAPDH/60004-1, Protein Tech, China) at room temperature for at least 1 hr. Then, the membrane was washed with PBST (PBS with 0.1% Tween 20) three times at an interval of 10 min. After washing, the membrane was incubated with the appropriate goat anti-rabbit (SE131, Solarbio, China) or goat anti-mouse (SE131, Solarbio, China) secondary antibodies at room temperature for 1 hr. After washing 6 times, the signals were visualized using the Immobilon Western Chemiluminescent HRP Substrate Kit (WBKLS0500, Millipore, Billerica, USA).
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