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Human annexin a1 elisa kit

Manufactured by Abcam
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The Human Annexin A1 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of Annexin A1 levels in human serum, plasma, and cell culture supernatants. The kit utilizes a specific antibody coated on a 96-well plate to capture Annexin A1 from the samples, and a detection antibody to quantify the captured protein.

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Lab products found in correlation

Spark microplate reader, by Tecan (1 mentions) Human il 1β elisa kit, by Abcam (1 mentions) Hmgb1 elisa kit exp, by Shino-Test (1 mentions)

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ELISAs (10 citations) Annexin A1 (6 citations)

3 protocols using human annexin a1 elisa kit

1

Annexin A1 ELISA Protocol

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Plasma AnxA1 levels were measured by ELISA (Human Annexin A1 ELISA Kit, ab222868, Abcam, Cambridge, UK) with specific polyclonal anti-human AnxA1 antibodies. The assays were conducted according to the manufacturer’s recommendations.
Santana B.B., Queiroz M.A., Cerveira R.A., Rodrigues C.M., da Silva Graça Amoras E., da Costa C.A., de Sousa M.S., Ishak R., Goulart L.R, & Vallinoto A.C. (2021). Low Annexin A1 level in HTLV-1 infected patients is a potential biomarker for the clinical progression and diagnosis of HAM/TSP. BMC Infectious Diseases, 21, 219.
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2

Detecting ANXA1 in Breast Cancer Cells

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To detect ANXA1 in conditioned medium (CM) of wild-type (WT), scramble or ERα and/or PPARα silenced MCF7 cells, the human Annexin A1 ELISA kit was used (ab222868, Abcam). Briefly, cells seeded in equal numbers in 12-well plates and silenced as previously described. CM was collected after 72 hours and used for ELISA assay. Absorbance was read at 450 nm with the Spark microplate reader (TECAN). The standard curve was used to define the protein concentration.
Cioni B., Ratti S., Piva A., Tripodi I., Milani M., Menichetti F., Langella T., Botti L., De Cecco L., Chiodoni C., Lecis D, & Colombo M.P. (2023). JMJD6 Shapes a Pro-tumor Microenvironment via ANXA1-Dependent Macrophage Polarization in Breast Cancer. Molecular Cancer Research, 21(6), 614-627.
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3

Quantifying Inflammation Biomarkers

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The concentrations of HMGB1, HSP70, annexin A1, and IL‐1β in culture supernatants were measured using an HMGB1 ELISA Kit Exp (Shino‐Test), an HSP70 ELISA Kit (Proteintech), a Human Annexin A1 ELISA Kit (Abcam), and a Human IL‐1β ELISA Kit (Abcam), respectively.
Okamura K., Inoue H., Tanaka K., Ikematsu Y., Furukawa R., Ota K., Yoneshima Y., Iwama E, & Okamoto I. (2022). Immunostimulatory oncolytic activity of coxsackievirus A11 in human malignant pleural mesothelioma. Cancer Science, 114(3), 1095-1107.
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