General reagents were certified ACS reagent grade and were purchased from Sigma-Aldrich (St. Louis, MO) and Fisher Scientific (Itasca, IL). Media and fetal bovine serum (FBS) were purchased from Fisher Scientific (Itasca, IL). HAL standards were purchased from Sigma-Aldrich, CanSyn Chem. Corp. (Toronto, ON), Aldlab Chemicals (Woburn, MA), and TCI America (Waltham, MA) at the highest level of purity available (chemical properties, purity, and CAS numbers of investigated HALs are provided in Supporting Information (SI), Table S1 ). 4-Fluorobenzaldehyde and 1,2-dibromopropane, used as the surrogate standard (SS) and internal standard (IS), respectively, and O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBHA), used as the derivatizing agent, were purchased from Sigma-Aldrich. Oasis HLB cartridges (6 cc, 150 mg, 30 µm particle size) for solid-phase extraction (SPE) were purchased from Waters (Milford, MA). All solvents (acetonitrile, n-hexane, methyl tert-butyl ether (MTBE), methanol, and ethyl acetate) were of highest purity and were purchased from Fisher Scientific, EMD Millipore (Billerica, MA) or VWR International (Radnor, PA).
1 2 dibromopropane
Manufactured by Merck Group
Sourced in Germany
1,2-dibromopropane is a chemical compound that is commonly used in laboratory settings. It has a molecular formula of C3H6Br2 and a molar mass of 201.88 g/mol. The core function of this compound is to serve as a versatile reagent in various organic synthesis reactions and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
N hexane, by Thermo Fisher Scientific (1 mentions)
4 fluorobenzaldehyde, by Merck Group (1 mentions)
O 2 3 4 5 6 pentafluorobenzyl hydroxylamine pfbha, by Merck Group (1 mentions)
Oasis hlb cartridge, by Waters Corporation (1 mentions)
General reagents, by Merck Group (1 mentions)
1 chlorodecane, by Merck Group (1 mentions)
Methanol, by Teknokroma (1 mentions)
Acetonitrile, by Teknokroma (1 mentions)
Iodoform, by Merck Group (1 mentions)
Acetone, by Teknokroma (1 mentions)
Bromoform, by Merck Group (1 mentions)
N octanol, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Iodoacetic acid, by Merck Group (1 mentions)
Methyl tert butyl ether (mtbe), by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
4 protocols using 1 2 dibromopropane
1
Quantitative Analysis of Halogenated Aromatics
2
Quantification of Disinfection By-Products
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Standards of THMs (bromoform, chloroform, bromodichloromethane, dibromochloromethane) and other neutral DBPs, viz. HANs (dichloroacetonitrile, dibromoacetonitrile, bromochloroacetonitrile, trichloroacetonitrile), HKs (1,1-dichloro-2-propanone and 1,1,1-trichloro-2-propanone) and a HNM (trichloronitromethane), and HAAs (monochloroacetic acid, monobromoacetic acid, dichloroacetic acid, dibromoacetic acid, trichloroacetic acid, bromochloroacetic acid, bromodichloroacetic acid, chlorodibromoacetic acid and tribromoacetic acid) were from Restek, acquired from Teknolab Sorbent (Kungsbacka, Sweden). Additional standards of 1,2-dibromopropane (97%) and 1-chlorodecane were acquired from Sigma-Aldrich (Stockholm, Sweden).
Stock solutions in methanol containing 0.2 μg μL−1 (stock solution 1, TableS1 ), 0.02 μg μL−1 (stock solution 2), and 0.002 μg μL−1 (stock solution 3) were prepared for every THM, HAN, HK, HNM, and HAA (see Table S1 in supplementary information for details). The stock solutions were added to samples of Milli-Q water for calibration, as well as to MtBE for direct GC-XSD determination. The surrogate standard 1,2-dibromopropane was prepared in methanol and the recovery standard 1-chlorodecane was prepared in MTBE.
Stock solutions in methanol containing 0.2 μg μL−1 (stock solution 1, Table
3
Determination of Iodo-Trihalomethanes in Water
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All reagents used were of highest purity, iodoform (CHI 3 ), 1,2 dibromopropane (used as internal standard SI) and THMs mixture 100 µg/µL of each compound: chloroform (CHCl 3 ), bromodicloromethane (CHBrCl 2 ), dibromochloromethane (CHBr 2 Cl), and bromoform (CHBr 3 ), were purchased from Sigma-Aldrich (Steinhein Germany). Iodo-trihalomethanes (I-THMs): bromodiiodomethane (CHBrI 2 90%), bromochloroiodomethane (CHBrClI 95%), chlorodiiodomethane (CHClI 2 95%), dibromoiodomethane (CHBr 2 I 95%) and dichloroiodomethane (CHCl 2 I 95%) were purchased from Orchid Cellmark (New Westminster, BC, Canada). Methanol, n-hexane, toluene, acetonitrile and acetone were purchased from Teknokroma (Barcelona, Spain). n-Octanol was supplied from Merck (Barcelona, Spain). Accurel Q 3/2 polypropylene hollow fiber membranes with an inner diameter of 600 µm, 200 µm of wall thickness and 0.2 µm pore size was obtained from Wuppertal (Germany). Ultrapure water (18MΩ cm) was obtained from a Milli-Q water-purification system (Millipore, Watford, UK) and was used for spiked samples and blanks preparation. to achieve a final concentration of 5 µg L -1 .
4
Haloacetic Acids Quantification Protocol
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A mixed standard containing MBAA, BCAA, bromodichloroacetic acid (BDCAA), MCAA, DBCAA, DBAA, DCAA, TBAA, and TCAA (EPA 552.2 Haloacetic Acids Mix, 2000 mg ml À1 each component in MTBE, >99%) was obtained from Sigma Aldrich (Saint-Quentin Fallavier, France). Iodoacetic acid (98%), 1,2-dibromopropane (internal standard, 97%) and sulfuric acid were also purchased from Sigma Aldrich, as well as L-ascorbic acid (99%) for neutralization of residual oxidants. DIAA (90%) was supplied from Santa Cruz Biotechnology (Heidelberg, Germany). 2,3-dibromopropionic acid (surrogate, 1000 mg ml À1 in MTBE) was obtained from Dr Ehrenstorfer GmbH (Augsburg, Germany). Methanol (MeOH, HPLC grade, 99.8%) and methyl-tert-butylether (MTBE, GC grade, 99.8%) were purchased from Merck (Darmstadt, Germany).
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