ChIP was performed with Agarose ChIP Kit (Pierce) and antibodies toward STAT3 (D3Z2G, 1:100), STAT6 (D3H4, 1:100), c-Jun (AP-1, 1:100) and NF-κB p65 (D14E12, 1:100) (all obtained from Cell Signaling Technology), C/EBPβ (C-190, 1:100) (Santa Cruz Biotechnology) and the respective isotype control antibodies on 2 × 106 cells starved for 3 hr and treated with medium alone or with IL-4 (10 ng/ml, 2 hrs). The DNA from sample and input was amplified by RT-PCR (40 cycles), using the following primers specific for the Il6 promoter; 5’-AGTGGTGAAGAGACTCAGTG-3’ (forward) and 5’-GGCAGAATGAGCCTCAGA-3’ (reverse) and specific primers for the Ccl11 promoter; 5’-CTTCATGTTGGAGGCTGAAG-3’ (forward) and 5’-GGATCTGGAATCTGGTCAGC-3’ (reverse). Results presented as the ratio of the cycling threshold value of immunoprecipitated DNA to that of input DNA.
C ebpβ c 190
Manufactured by Santa Cruz Biotechnology
C/EBPβ (C-190) is a laboratory reagent produced by Santa Cruz Biotechnology. It is a protein that functions as a transcription factor, regulating the expression of various genes involved in cellular processes such as differentiation, proliferation, and inflammation.
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Lab products found in correlation
2 protocols using c ebpβ c 190
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Transcription Factor Binding Assay
2
Transcription Factor Binding Assay
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ChIP was performed with Agarose ChIP Kit (Pierce) and antibodies toward STAT3 (D3Z2G, 1:100), STAT6 (D3H4, 1:100), c-Jun (AP-1, 1:100) and NF-κB p65 (D14E12, 1:100) (all obtained from Cell Signaling Technology), C/EBPβ (C-190, 1:100) (Santa Cruz Biotechnology) and the respective isotype control antibodies on 2 × 106 cells starved for 3 hr and treated with medium alone or with IL-4 (10 ng/ml, 2 hrs). The DNA from sample and input was amplified by RT-PCR (40 cycles), using the following primers specific for the Il6 promoter; 5’-AGTGGTGAAGAGACTCAGTG-3’ (forward) and 5’-GGCAGAATGAGCCTCAGA-3’ (reverse) and specific primers for the Ccl11 promoter; 5’-CTTCATGTTGGAGGCTGAAG-3’ (forward) and 5’-GGATCTGGAATCTGGTCAGC-3’ (reverse). Results presented as the ratio of the cycling threshold value of immunoprecipitated DNA to that of input DNA.
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