Ab7970

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Ab7970 is a lab equipment product offered by Santa Cruz Biotechnology. It is designed for research applications, but its core function is not available for detailed description while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ab109374, by Abcam (1 mentions) Sc 6243, by Santa Cruz Biotechnology (1 mentions) Sc 764, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti iκbα, by Santa Cruz Biotechnology (1 mentions) Sc 371, by Santa Cruz Biotechnology (1 mentions) Ab37415, by Abcam (1 mentions) P4170 10mg, by Merck Group (1 mentions) β actin a5441, by Merck Group (1 mentions) Novex tris glycine precast gels, by Thermo Fisher Scientific (1 mentions) Sc 146, by Abcam (1 mentions) Supersignal west pico chemiluminescent substrate system, by Thermo Fisher Scientific (1 mentions) Scion image software, by Techcomp Instruments (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Anti actin, by Santa Cruz Biotechnology (1 mentions)

4 protocols using ab7970

Quantification and Immunoblotting of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total and nuclear proteins were prepared and quantified as already reported [15 (link), 24 (link)]. Rabbit antibodies against p65 (Abcam, ab7970), p53 (Santa Cruz Biotechnology, sc-6243), c-Myc (Santa Cruz Biotechnology, sc-764), p-Akt S473 (Cell Signaling, 3787), and topoisomerase I (Abcam, ab109374), and mouse antibodies against β-actin (Sigma-Aldrich) and Akt1 (Cell Signaling, 2967) were used. Densitometry was performed with ImageJ software.

Porreca I., Ulloa Severino L., D’Angelo F., Cuomo D., Ceccarelli M., Altucci L., Amendola E., Nebbioso A., Mallardo M., De Felice M, & Ambrosino C. (2016). “Stockpile” of Slight Transcriptomic Changes Determines the Indirect Genotoxicity of Low-Dose BPA in Thyroid Cells. PLoS ONE, 11(3), e0151618.

+ Open protocol

+ Expand

Quantification and Western Blot Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein was quantified by a BCA Kit after cells were lysed in RIPA buffer as previously described (Duan et al., 2015 (link)). 20 μg of protein lysates were subjected to 10% SDS-PAGE and electrotransferred to a 0.22 μm PVDF membrane (Millipore). The membrane was incubated in primary antibody overnight at 4 °C and HRP-conjugated secondary antibody followed by visualization using chemi-luminescence. The antibodies used were as follows: anti-RelA (Abcam, ab7970, 1:1000), anti-IκBα (Santa Cruz, sc-371, 1:1000) and anti-β-Actin (Santa Cruz, sc-69879, 1:4000).

Wang P., Liu Z., Zhang X., Li J., Sun L., Ju Z., Li J., Chan P., Liu G.H., Zhang W., Song M, & Qu J. (2018). CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells. Protein & Cell, 9(11), 945-965.

+ Open protocol

+ Expand

Preparation and Use of AS-10 Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols

A stock solution of AS-10 was prepared by dissolving in DMSO at a 10 mM concentration, storing at –20 °C. Each stock solution was used within 7 days of preparation. The final DMSO concentration for all treatments was kept at 0.1%. Antibodies were ordered from the following commercial sources: Cell signaling (Danvers, MA, USA) (IκBα (9242s), Bcl-xL (2762s), Mcl-1 (5453s) and PARP (9542s)); Abcam (Cambridge, MA, USA) (P100/P50 (ab31412), GAPDH (5174p) and P65 (ab7970)); Santa Cruz Biotechnology (Dallas, TX, USA) (P21 (sc-397); Jackson Immuno Research (West Grove, PA, USA) (Alexa fluor 680 (711-625-152) and donkey serum (017-000-002)); and Sigma-Aldrich (St. Louis, MO, USA) (β-actin (a-5441)). Rabbit IgG-chip grade protein was ordered from Abcam (ab37415), while propidium iodide (P4170-10MG), and ribonuclease A (RNAse A, R6513-10MG) and crystal violet (C6158-50G) from Sigma-Aldrich. Gemzar (gemcitabine) was obtained from Penn State Hershey Medical Center pharmacy (Hershey, PA, USA).

Karelia D.N., Kim S., K. Pandey M., Plano D., Amin S., Lu J, & Sharma A.K. (2021). Novel Seleno-Aspirinyl Compound AS-10 Induces Apoptosis, G1 Arrest of Pancreatic Ductal Adenocarcinoma Cells, Inhibits Their NF-κB Signaling, and Synergizes with Gemcitabine Cytotoxicity. International Journal of Molecular Sciences, 22(9), 4966.

+ Open protocol

+ Expand

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brain tissue were washed in phosphate-buffered saline once and sonicated in lysis buffer (20 mM Tris pH 7.6, 100 mM NaCl, 1% Nonidet P-40, 0.1% SDS, 1% deoxycholic acid, 10% glycerol, 1 mM EDTA, 1 mM NaVO3, 50 mM NaF, Protease Inhibitor Cocktail Set I from Calbiochem). Soluble protein was then obtained by centrifugation at 13,000 × g at 4°C for 15 minutes. Bicinchoninic acid (BCA) protein assay (Pierce) was used to measure total protein concentration. Equal amounts of lysate (20 μg protein/lane) were loaded to SDS-polyacrylamide electrophoresis on Novex Trisglycine precast gels (Invitrogen), and separated proteins were then electrotransferred to polyvinylidene fluoride (PVDF) membranes (Millipore). SuperSignal West Pico Chemiluminescent Substrate system (Thermo Scientific) was applied to visualize different proteins by reacting to various antibodies. The band intensity was analyzed using Scion Image software (Scion). Antibodies used for Western blot included anti-tumor transforming growth factor beta1 (TGF-β1) polyclonal antibody (Santa Cruz, SC-146); anti-nuclear factor-kappa B (NFkB) antibody (Abcam, ab7970) and anti-actin (1:5000, Santa Cruz Biotechnology).

Zhang Y., Zhang Z.G., Chopp M., Meng Y., Zhang L., Mahmood A, & Xiong Y. (2016). Treatment of traumatic brain injury in rats with N-acetyl-seryl-aspartyl-lysyl-proline. Journal of neurosurgery, 126(3), 782-795.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!