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Il 4 quantikine elisa kit

Manufactured by R&D Systems

The IL-4 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human interleukin 4 (IL-4) levels in cell culture supernates, serum, and plasma. It utilizes a microplate precoated with a monoclonal antibody specific for IL-4.

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2 protocols using il 4 quantikine elisa kit

1

Quantifying Cytokine Profiles in IMQ-Induced Mouse Model

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Total protein extracted from P155- or Scr-treated IMQ mouse ears was quantified with the BCA kit (Thermo Fisher Scientific). Protein expression of IL-17A, IFN-γ, and IL-4 was measured using mouse IL-17A (BioLegend) enzyme-linked immunosorbent assay (ELISA) Ready-SET-Go kit, mouse IFN-γ, and IL-4 quantikine ELISA kit (R&D Systems) according to the instructions.
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2

ELISA Quantification of Cytokines

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Enzyme-linked immunosorbent assay (ELISA) for IL-33 and sST2 in serum was performed using the IL-33 Quantikine ELISA Kit (R&D Systems, Inc.) and the sST2 Quantikine ELISA Kit (R&D Systems, Inc.). The supernatant of the cells was collected and centrifuged at 4°C at 1,000 g for 10 min. IL-4 Quantikine ELISA kit (R&D Systems, Inc.) and IL-6 Quantikine ELISA kit (R&D Systems, Inc.) were used to measure the protein secretion of IL-4 and IL-6 in esophageal adenocarcinoma cells following the manufacturer’s instructions.
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