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Anti brd4

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Anti-BRD4 is a laboratory reagent designed to detect and quantify the presence of the BRD4 protein in biological samples. BRD4 is a member of the bromodomain and extraterminal (BET) family of proteins and plays a key role in the regulation of gene expression. The Anti-BRD4 reagent can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the expression and function of the BRD4 protein.

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Lab products found in correlation

A11029, by Thermo Fisher Scientific (2 mentions) Anti cstf64, by Thermo Fisher Scientific (2 mentions) Anti γh2ax for in vitro if, by Merck Group (2 mentions) Anti γh2ax for mif, by Cell Signaling Technology (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti panck, by Abcam (2 mentions) Anti brdu, by BD (2 mentions) Anti p27, by Abcam (2 mentions) Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Ripa buffer, by Merck Group (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Abcam (1 mentions)

3 protocols using anti brd4

1

BRD4 Protein Quantification by Western Blot

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Cells were lysed with RIPA buffer (Sigma-Aldrich) containing protease inhibitor cocktail (Thermo Fisher Scientific). Protein concentration was determined by a commercial BCA assay kit (Sigma-Aldrich). The cell extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The following primary antibodies were used: anti-BRD4 (1:1000 dilution; Thermo Fisher Scientific) and anti-GAPDH (1:5000 dilution; Abcam). Horseradish peroxidase-conjugated IgG was used as secondary antibody. The blots were developed using the Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific). Densitometric analysis of protein bands was then performed.
Fan H., Yuan J., Li Y., Jia Y., Li J., Wang X, & Li X. (2021). MKL1-induced lncRNA SNHG18 drives the growth and metastasis of non-small cell lung cancer via the miR-211-5p/BRD4 axis. Cell Death & Disease, 12(1), 128.
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2

Antibody Panel for Multimodal Protein Analysis

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Primary antibodies used in this study are anti-Top1 (Abcam, ab109374), anti-BRD4 (Thermo Fisher Scientific, A301985A), anti-H3K36me3 (Abcam, ab9050), anti-H3K27ac (Abcam, ab177178), anti-CSTF64 (Thermo Fisher Scientific, A301092A), anti–RNAPII–Ser2-P (Abcam, ab5095), anti–total RNAPII (Abcam, ab817), anti-γH2AX for in vitro IF (Sigma-Aldrich, 05-636), anti-γH2AX for mIF (Cell Signaling Technology, 9718S), anti-p27 (Abcam, ab32034), anti-BrdU (Becton Dickinson, 555627), anti–cleaved caspase 3 (Cell Signaling Technology, 9664 L), and anti-PanCK (Abcam, ab6401). Secondary antibodies are anti-mouse (Thermo Fisher Scientific, A11029) and anti-rabbit (Thermo Fisher Scientific, A32790) Alexa Fluor 488–conjugated antibodies.
Cameron D.P., Grosser J., Ladigan S., Kuzin V., Iliopoulou E., Wiegard A., Benredjem H., Jackson K., Liffers S.T., Lueong S., Cheung P.F., Vangala D., Pohl M., Viebahn R., Teschendorf C., Wolters H., Usta S., Geng K., Kutter C., Arsenian-Henriksson M., Siveke J.T., Tannapfel A., Schmiegel W., Hahn S.A, & Baranello L. (2023). Coinhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer via readthrough transcription. Science Advances, 9(41), eadg5109.
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3

Antibody Panel for Multimodal Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used in this study are anti-Top1 (Abcam, ab109374), anti-BRD4 (Thermo Fisher Scientific, A301985A), anti-H3K36me3 (Abcam, ab9050), anti-H3K27ac (Abcam, ab177178), anti-CSTF64 (Thermo Fisher Scientific, A301092A), anti–RNAPII–Ser2-P (Abcam, ab5095), anti–total RNAPII (Abcam, ab817), anti-γH2AX for in vitro IF (Sigma-Aldrich, 05-636), anti-γH2AX for mIF (Cell Signaling Technology, 9718S), anti-p27 (Abcam, ab32034), anti-BrdU (Becton Dickinson, 555627), anti–cleaved caspase 3 (Cell Signaling Technology, 9664 L), and anti-PanCK (Abcam, ab6401). Secondary antibodies are anti-mouse (Thermo Fisher Scientific, A11029) and anti-rabbit (Thermo Fisher Scientific, A32790) Alexa Fluor 488–conjugated antibodies.
Cameron D.P., Grosser J., Ladigan S., Kuzin V., Iliopoulou E., Wiegard A., Benredjem H., Jackson K., Liffers S.T., Lueong S., Cheung P.F., Vangala D., Pohl M., Viebahn R., Teschendorf C., Wolters H., Usta S., Geng K., Kutter C., Arsenian-Henriksson M., Siveke J.T., Tannapfel A., Schmiegel W., Hahn S.A, & Baranello L. (2023). Coinhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer via readthrough transcription. Science Advances, 9(41), eadg5109.
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