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Rhein is a laboratory equipment used for the analysis and separation of chemical compounds. It utilizes high-performance liquid chromatography (HPLC) technology to facilitate the identification and purification of target substances. The core function of Rhein is to provide accurate and efficient separation and characterization of complex mixtures, enabling researchers to study the chemical composition and properties of various samples.

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2 protocols using rhein

1

Aspirin and Herbal Compound ELISA Protocol

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Aspirin was purchased from by Bayer HealthCare with the batch number of J20130078). Enzyme-linked Immunosorbent Assay (ELISA) Kits including Rat interleukin 1 (IL-1), Rat S-100B protein, Rat nuclear factorκB (NF-κB), Rat neuron specific enolase (NSE), Rat high sensitive C reactive protein (HS-CRP), Rat estradiol (E2), Rat tumor necrosis factor-α (TNF-α), and Rat D-dimmer (D2D), were purchased from Nanjing Senbeijia Biological Technology Co., Ltd., Trizol was purchased from Invitrogen. Anti-AKT, Anti-PI3K, and Anti-β-actin antibody was all purchased from Abcam. All durgs (purity assay by HPLC ≥ 98%, power) including compounds of Emodin, Ginsenoside Rg1, Ginsenoside Rb1, Notoginsenoside R1, Baicalein, Geniposide, β-asarone, Taurine, Paeoniflorin, Paeonol, Apigenin, Rhein, and Catalpol, were purchased from Chengdu Must Bio-Technology Co., Ltd.
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2

Simultaneous Quantification of Seven Compounds in SK Injection

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Authentic standards of gallic acid, danshensu, rosmarinic acid, salvianolic acid A, aloe-emodin, rhein and emodin were obtained from Chengdu Must Bio-technology Co., Ltd. (Sichuan, China). Carbamazepine using as internal standard was purchased from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). These compounds were determined with the purity >98% by HPLC or GC method. The medicines of SK injection were bought from Xian Shiji Shengkang Pharmaceutical Industry Co., Ltd. (Xian, Shannxi, China).
Methanol and acetonitrile (chromatographic grade) were purchased from Fisher Scientific (Pittsburgh, PA, USA). LC-MS grade formic acid was obtained from Aladdin Industrial Co., Ltd. (Shanghai, China). All the other chemicals were analytical grade. The deionized water (18.2 MΩ) was purified by a Millipore water purification system (Millipore, Shanghai, China) and all of the solutions were filtrated by 0.22 μm pore size filters.
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