Human cervical cancer cell line (HeLa) was obtained from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), trypsin, and phosphate-buffered solution (PBS, pH 7.4) were all purchased from Hyclone (San Angelo, TX, USA). Taxol and PTX were donated by Beijing ShuangLu Pharmaceutical Co, Ltd. Peptides and fluorescein isothiocyanate (FITC)-labeled peptides were synthesized by GL Biochem (Shanghai, People’s Republic of China). Chlorpromazine (CPZ) was purchased from Heowns Biochemical Technology (Tianjin, People’s Republic of China), nystatin (Nys) from Topscience (Shanghai, People’s Republic of China), 2-deoxy-d -glucose (2-D-G) and dimethyl sulfoxide (DMSO) from Aladdin (Shanghai, People’s Republic of China), sodium azide (NaN3) from Micxy Chemical Co (Cheng Du, People’s Republic of China), paraformaldehyde (PFA), 4′,6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and heparin from Solarbio (Beijing, People’s Republic of China). Mouse anti-β-tubulin primary antibody and FITC-labeled goat anti-mouse secondary antibody were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Female BALB/c mice were purchased from Beijing HFK Bioscience Co, Ltd (Beijing, People’s Republic of China).
Fitc labeled goat anti mouse secondary antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
FITC-labeled goat anti-mouse secondary antibody is a laboratory reagent used to detect and visualize mouse primary antibodies in various immunological techniques, such as Western blotting, immunohistochemistry, and flow cytometry. The antibody is conjugated with the fluorescent dye FITC, which allows for fluorescence-based detection of the target antigen.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde (pfa), by Solarbio (1 mentions)
Mouse anti β tubulin primary antibody, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Heparin, by Solarbio (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Solarbio (1 mentions)
2 protocols using fitc labeled goat anti mouse secondary antibody
1
Cytotoxicity Evaluation of Taxol Analogs
2
Glycosylation and Phosphorylation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fmoc-Tyr(PO(NMe 2 ) 2 )-OH was purchased from Merck Millipore. Fmoc-Ser(β-d-GlcNAc(Ac) 3 )-OH was synthesized in our laboratory according to a literature procedure (Sharif et al. 2017) (link). Primary antibody mouse monoclonal Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) was purchased from Abcam (London, England) and FITC labeled goat anti-mouse secondary antibody was obtained from Thermo Scientific (Bleiswijk, the Netherlands). PamChip4 microarray chips, fluorescein-labeled PY20 antibody, PTP 1B, AP, SHIP1, SHIP2 and PTP buffer were provided by PamGene (Pamgene International B.V., 's-Hertogenbosch, The Netherlands). O-GlcNAcase (OGA) was overexpressed as previously reported (Sharif et al. 2017) (link).
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