Euryx simple save

The specimen details were as follow: Gymnomitrion concinnatum (Lightf.) Corda, Slovakia, High Tatra Mountains, Výsňé Kôprovické sedlo pass (Liptovské kopy), 49.19040°N, 19.96641°E, alt. 1910 m a.s.l., fine-grained screen with high participation of lichens, leg., det. P. Górski, 4.09.2014. The DNA was extracted using the Zymo Plant/Seed DNA kit (Zymo Research, Irvine, CA, USA). One individual from a one year old herbarium specimen was ground with silica beds in a MiniBead-Beater homogenizer for 50 s and subsequently processed according to the manufacturer protocol. DNA quantity was estimated using Qubit fluorometer and Qubit™ dsDNA BR Assay Kit (Initrogen, Carsbad, NM, USA). DNA quality was checked by electrophoresis in 0.5% agarose gel stained with Euryx Simple Save (Eurx, Gdańsk, Poland).

The total genomic DNA of liverworts were extracted from fresh tissue stored in−20 °C. The DNA was extracted using the Qiagen Plant MiniSpin (Qiagen, Germany). Ca 1 cm² of cleaned thallus was ground with silica beads in a MiniBead-Beater homogenizer for 50 s and subsequently processed according to the manufacturer protocol. The DNA of Riccia fluitans and Sphaerocarpos texanus were extracted from 1 cm long thalli from herbarium specimens.
The DNA quantity was estimated using Qubit fluorometer and Qubit™ dsDNA BR Assay Kit (Invitrogen, Carsbad, NM, USA). DNA quality was checked by electrophoresis in 0.5% agarose gel that was stained with Euryx Simple Save (Eurx, Gdańsk, Poland). Table S1 provides sample specimen details.
The extracted DNA of Conocephalum species prior to nanopore sequencing was carefully examined and additionally cleaned while using Genomic DNA Clean and Concentrator kit (Zymo, Irvine, CA, USA).