Log In Sign up
The largest database of trusted experimental protocols

Neurobasal medium without phenol red

Manufactured by Thermo Fisher Scientific
Visit Supplier

Neurobasal medium without phenol red is a cell culture medium designed for the growth and maintenance of neuronal cells. It is a serum-free, chemically defined medium that supports the survival and differentiation of neurons in culture. The medium does not contain the pH indicator phenol red, which can interfere with certain experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Neurobasal electro medium, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Neurobasal medium (2692 citations) Neurobasal (586 citations) Phenol red (557 citations) Phenol (68 citations)

2 protocols using neurobasal medium without phenol red

1

Oxidative Stress Response in BrainSpheres

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 4 weeks of differentiation, the medium was replaced with 2 ml of fresh differentiation medium (Fig. 1A). Subsequently, BrainSpheres were exposed for 24 h to compounds known to induce ROS production (6-OHDA, MPTP, or MPP+) at a wide range of concentrations (10 μM – 5000 μM) (Fig. 1A). For the ROS assay, differentiation medium Neurobasal® electro medium [Gibco] was replaced with Neurobasal medium without phenol red [Gibco]. The 6-wells plates were returned to the incubator with gyratory shaking (5.0% CO2, 37 °C) for 24 h. After exposure, multiple assays were performed as described below. In addition, in order to study the recovery of the cells, different treatments were removed from the media, BrainSpheres were washed 3 times with PBS, and then cultured for 5 days before collection.
Pamies D., Wiersma D., Katt M.E., Zhao L., Burtscher J., Harris G., Smirnova L., Searson P.C., Hartung T, & Hogberg H.T. (2022). Human IPSC 3D brain model as a tool to study chemical-induced dopaminergic neuronal toxicity. Neurobiology of disease, 169, 105719.
+ Open protocol
+ Expand
2

Oxidative Stress Response in BrainSpheres

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 4 weeks of differentiation, the medium was replaced with 2 ml of fresh differentiation medium (Fig. 1A). Subsequently, BrainSpheres were exposed for 24 h to compounds known to induce ROS production (6-OHDA, MPTP, or MPP+) at a wide range of concentrations (10 μM – 5000 μM) (Fig. 1A). For the ROS assay, differentiation medium Neurobasal® electro medium [Gibco] was replaced with Neurobasal medium without phenol red [Gibco]. The 6-wells plates were returned to the incubator with gyratory shaking (5.0% CO2, 37 °C) for 24 h. After exposure, multiple assays were performed as described below. In addition, in order to study the recovery of the cells, different treatments were removed from the media, BrainSpheres were washed 3 times with PBS, and then cultured for 5 days before collection.
Pamies D., Wiersma D., Katt M.E., Zhao L., Burtscher J., Harris G., Smirnova L., Searson P.C., Hartung T, & Hogberg H.T. (2022). Human IPSC 3D brain model as a tool to study chemical-induced dopaminergic neuronal toxicity. Neurobiology of disease, 169, 105719.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!