Terminal deoxynucleotidyl transferase mediated dutp nick end labeling tunel apoptosis detection kit

BCF was obtained from the Department of Pharmacology of Guilin Medical University, Guilin, Guangxi, People’s Republic of China.23 With rutin as a reference substance, the total flavonoid content of BCF was 82% (Figure S1). BCF was diluted with saline to an appropriate concentration as needed. Creatine kinase-MB (CK-MB) and nitric oxide (NO) detection kits were obtained from the Jiancheng Bioengineering Institute (Nanjing, People’s Republic of China). MPTP, TNF-α, and enzyme-linked immunosorbent assay kits were purchased from Chenglin Biological Technology Co. Ltd. (Beijing, People’s Republic of China). A terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) apoptosis detection kit was purchased from Roche Diagnostics (Mannheim, Germany). Primers were purchased from Takara Biotechnology Co. Ltd. (Dalian, People’s Republic of China). WM was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). All antibodies were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). The extraction and isolation of BCF and each chemical structure of BCF constituents are in the Supplementary material.

Terminal deoxynucleotidyl transferase-mediated dUTP-nick end-labeling (TUNEL) apoptosis detection kit (Roche, Indianapolis, IN, USA), rabbit anti-Bcl-2 antibody, rabbit anti-Bax antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA); rabbit anti-mouse β-actin primary antibody (Santa Cruz Biotechnology) and horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (Boaosen, Beijing, China). Twenty-four adult male Wistar rats (body weight 280–350 g) were purchased from the Animal Laboratory Center of Tianjin Medical University (Tianjin, China).