Mabc1640

Manufactured by Merck Group

Sourced in United States

MABC1640 is a laboratory equipment product by Merck Group. It is a multi-purpose device designed for various scientific applications. The core function of MABC1640 is to facilitate precise measurements and data collection in a laboratory setting.

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Lab products found in correlation

Sc 47778 hrp, by Santa Cruz Biotechnology (2 mentions) Ab187091, by Abcam (2 mentions) Mabc604, by Merck Group (2 mentions) Mab374, by Merck Group (1 mentions) Mabc1636, by Merck Group (1 mentions) Epr9627, by Abcam (1 mentions)

2 protocols using mabc1640

Antibodies and Reagents for MLKL and RIPK3

Cited 1 time

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Primary antibodies used in this study were: rat anti-MLKL (clone 3H1, produced in-house; 1:1000 dilution; available as MABC604, EMD Millipore, Billerica, MA, USA), rat anti-human MLKL pseudokinase domain (clone 7G2, produced in-house^{32 (link)}; 1:2000 dilution; soon available from EMD Millipore as MABC1636), rabbit anti-human MLKL phospho-S358 (AB187091, Abcam; 1:2000), mouse anti-Actin (C4) HRP (sc-47778 HRP, Santa Cruz Biotechnology; 1:10000), rat anti-human RIPK3 (clone 1H2, produced in-house^{11 (link)}; 1:1000; available as MABC1640, EMD Millipore, Billerica, MA, USA), rabbit anti-human RIPK3 phospho-S227 (D6W2T, CST; 1:2000). Recombinant hTNF-Fc^{62 (link)} was produced in-house, while the Smac mimetic, Compound A^{63 (link)} and the pan-caspase inhibitor, IDN-6556/Emricasan, were provided by Tetralogic Pharmaceuticals. The RIPK3 inhibitors, GSK′843 (ref. ^{54 (link)}) and BMS Compound 10 (ref. ^{48 (link)}), were kindly provided by Anaxis Pty Ltd (Australia).

Meng Y., Davies K.A., Fitzgibbon C., Young S.N., Garnish S.E., Horne C.R., Luo C., Garnier J.M., Liang L.Y., Cowan A.D., Samson A.L., Lessene G., Sandow J.J., Czabotar P.E, & Murphy J.M. (2021). Human RIPK3 maintains MLKL in an inactive conformation prior to cell death by necroptosis. Nature Communications, 12, 6783.

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Immunoblotting of Necroptosis Mediators

Cited 1 time

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Primary antibodies used in this study were: rat anti-human RIPK3 (clone 1H2, produced in-house; 1:1000; available as MABC1640, EMD Millipore, Billerica, MA, USA) [25 (link)], rat anti-mouse RIPK3 (clone 1H12, produced in-house; 1:1000) [41 (link)], rabbit anti-human RIPK3 phospho-S227 (D6W2T, CST, 1:2000), rabbit anti-human RIPK3 phospho-S227 (EPR9627, Abcam; 1:2000) rat anti-human MLKL pseudokinase domain (clone 7G2, produced in-house; 1:2000 dilution; available as MABC1636, EMD Millipore, Billerica, MA, USA) [27 (link)], rat anti-human MLKL (3H1, produced in-house; 1:1000; available as MABC604, EMD Millipore, Billerica, MA, USA) [25 (link)], mouse anti-human RIPK1 (610459, BD Transduction Laboratories; 1:1000), rabbit anti-human MLKL phospho-S358 (AB187091, Abcam; 1:2000), mouse anti-GAPDH (MAB374, Millipore; 1:2000) and anti-Actin (C4) HRP (sc-47778 HRP, Santa Cruz Biotechnology; 1:10,000). The Smac mimetic, Compound A [42 (link)] and the pan-caspase inhibitor, IDN-6556/Emricasan, were provided by Tetralogic Pharmaceuticals, and recombinant hTNF-Fc [43 (link)] was produced in-house.

Meng Y., Horne C.R., Samson A.L., Dagley L.F., Young S.N., Sandow J.J., Czabotar P.E, & Murphy J.M. (2022). Human RIPK3 C-lobe phosphorylation is essential for necroptotic signaling. Cell Death & Disease, 13(6), 565.

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