MRI was performed on a Bruker Biospec 9.4 T animal system with Bruker BGA12S-HP gradients (660 mT/m) using a 76 mm quadrature volume coil (Bruker Biospin, Ettlingen, Germany). Anatomical scans were performed with a RARE sequence with the following parameters; Echo time (TE) of 20 ms, RARE factor 8, 16 averages and a repetition time (TR) of 15204.8 ms. The slice thickness was 0.25 mm, in-plane resolution was 0.125 x 0.125 mm2 and the total number of slices was 80. Scan time for the anatomical data acquisition was 2 hrs 38 min.
Diffusion data was acquired with the 1-9-9 method outlined above and in [28 ]: 1 image with b = 0 ms/μm2, 9 images with b = 1 ms/μm2 and 9 images with b = 2.5 ms/μm2. The dMRI data was acquired with the following imaging parameters: TE = 27.387 ms, TR = 7500 ms, and diffusion timings δ/Δ = 6/14 ms. Data was acquired with an in-plane resolution of 0.25 x 0.25 mm2, and a slice thickness of 0.5 mm. Whole brain data was acquired (30 slices in total) with two averages. Scan time for the DKI protocol was 14 hours.
Diffusion data was acquired with the 1-9-9 method outlined above and in [28 ]: 1 image with b = 0 ms/μm2, 9 images with b = 1 ms/μm2 and 9 images with b = 2.5 ms/μm2. The dMRI data was acquired with the following imaging parameters: TE = 27.387 ms, TR = 7500 ms, and diffusion timings δ/Δ = 6/14 ms. Data was acquired with an in-plane resolution of 0.25 x 0.25 mm2, and a slice thickness of 0.5 mm. Whole brain data was acquired (30 slices in total) with two averages. Scan time for the DKI protocol was 14 hours.