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2 protocols using rabbit anti aif

1

Synthesis and Characterization of HA-DPA-β-lap Conjugate

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N-hydroxy succinimide (NHS) and 3,3′-dithiodipropionic acid (DPA) were purchased from Sigma-Aldrich (Beijing, China). N,N-Dicyclohexylcarbodiimide (DCC) were purchased from Alfa Aesar (Shanghai, China). Hyaluronic acid (HA) was purchased from Macklin (Shanghai, China). N,N-Dimethylformamide (DMF) and dimethyl sulfoxide (DMSO) were purchased from Aladdin (Shanghai, China), β-lapachone (abbreviated as β-lap) was provided from yuanye Bio-Technology (Shanghai, China). Primary antibodies including rabbit anti-BID, rabbit anti-AIF, rabbit anti-EndoG, mouse anti-GAPDH and mouse anti-GPX4 were brought from Proteintech (Wuhan, China). Cell counting kit-8 (CCK-8) was purchased from Sevenbio (Beijing, China). Hoechst 33342 staining solution and mitochondrial membrane potential assay kit with JC-1 were purchased from Beyotime Biotechnology (Shanghai, China), reactive oxygen species assay kit and Fluo-4 AM probe were brought from Solarbio Science & Technology Co., Ltd. (Beijing, China).
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2

Cell Culture Reagents and Antibodies

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Cell culture reagents were purchased from Life Technologies (Shanghai, China). Rabbit anti-GAPDH and mouse anti-CELF1 were purchased from Santa Cruz Biotechnology (Shanghai, China). Mouse anti-Ecadherin was purchased from BD (Shanghai, China). Rabbit anti-caspase-3, 7, and cleaved caspase-3, 7 were purchased from CST (Shanghai, China). Rabbit anti-AIF was purchased from Proteintech (Shanghai, China). Horseradish peroxidase conjugated anti-mouse and anti-rabbit IgG were purchased from CST (Shanghai, China). All other chemicals were purchased from Sigma (Shanghai, China).
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