The following antibodies were used for adhesion blockade: for integrin α5 subunit (clone HMα5–1, Santa Cruz Biotechnology, Santa Cruz, CA), for integrin αv subunit (clone H9.2B8, Santa Cruz Biotechnology), and for integrin α9 subunit (antibody was supplied by Dr. Yasuyuki Yokosaki and generated in a similar manner as previously described) [24 (link)]. For immunostaining, antibodies used were against extra domain A (EIIIA) (clone IST9; Santa Cruz Biotechnology) (1:200), total fibronectin (Santa Cruz Biotechnology) (1:200), Ki-67 (Abcam) (1:200) and VE cadherin (R & D Systems, Minneapolis, MN) (1:200). For immunoblotting, antibodies used were against GAPDH (Abcam, Cambridge, MA) (1:10,000), total fibronectin (Santa Cruz Biotechnology) (1:1000), LYVE1 (Abcam)(1:100) and VEGFR3 (Abcam)(1:100).
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