T98G cells were dissociated with 0.25% trypsin to prepare a cell suspension. The suspension was filtered through a cell strainer to remove cell clusters. The total cell number was then counted. The single‐cell suspension was centrifuged at 600 rpm for 1‐2 minutes at 4°C to remove the supernatant. The cell pellet was then resuspended in 5 mL of DMEM supplemented with 10% FBS. TMZ (500 μmol/L) and/or FM19G11 (300 nmol/L) was then added to the cells, followed by 2 hours of incubation at 37°C. The cells were then washed with cold PBS and stained with an Annexin V‐FITC/propidium iodide solution (BD Biosciences, CA, USA). The samples were analyzed on a flow cytometer (Beckman Coulter, Brea, CA, USA) using a 488‐nm excitation wavelength.
Annexin 5 fitc propidium iodide solution
Manufactured by BD
Sourced in United States
Annexin V-FITC/propidium iodide solution is a laboratory reagent used for the detection and quantification of apoptosis (programmed cell death) in cell samples. Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine, which is exposed on the outer membrane of apoptotic cells. Propidium iodide is a fluorescent dye that binds to DNA and is used to identify necrotic or late-stage apoptotic cells. The combination of these two components allows for the differentiation between viable, early apoptotic, and late apoptotic/necrotic cells in a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5 fitc propidium iodide solution
1
Apoptosis Induction in T98G Cells
2
Apoptosis and Proliferation Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The apoptotic cells in cultures were detected using the Annexin V-FITC/propidium iodide method. Cells were incubated with Annexin V-FITC/propidium iodide solution (BD Biosciences, Franklin Lakes, NJ) for 15 minutes at room temperature. After washing, cells were analyzed by using an LSRII instrument (BD FACSCalibur, Franklin Lakes, NJ). Similarly, proliferating cells were detected using Alexa Fluor 488econjugated rabbit anti-Ki-67 IgG (Cell Signaling).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!