Tissues frozen in liquid nitrogen were collected, and RNA was extracted with on miRNeasy FFPE kit (Qiagen). MiRcute enhanced miRNA cDNA rst strand synthesis Kit (TIANGEN) was used to reverse transcribe the RNA into complementary DNA. The qRT-PCR analysis was performed using the qPCR miRcute enhanced miRNA uorescence quanti cation kit (TIANGEN). The results were normalised to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. We conducted three replicate experiments. The primers were synthesized by Thermo sher (Table S1).
Qpcr mircute enhanced mirna uorescence quanti cation kit
Manufactured by Tiangen Biotech
The QPCR miRcute enhanced miRNA fluorescence quantification kit is a laboratory equipment product designed for the quantitative detection and analysis of microRNA (miRNA) expression levels. The kit utilizes real-time quantitative PCR (qPCR) technology to provide a sensitive and accurate method for measuring miRNA abundance.
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2 protocols using qpcr mircute enhanced mirna uorescence quanti cation kit
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Quantitative miRNA Expression Analysis
2
Quantitative miRNA Expression Analysis
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Tissues frozen in liquid nitrogen were collected, and RNA was extracted with on miRNeasy FFPE kit (Qiagen). MiRcute enhanced miRNA cDNA rst strand synthesis Kit (TIANGEN) was used to reverse transcribe the RNA into complementary DNA. The qRT-PCR analysis was performed using the qPCR miRcute enhanced miRNA uorescence quanti cation kit (TIANGEN). The results were normalised to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. We conducted three replicate experiments. The primers were synthesized by Thermo sher (Table S1).
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