The metabolites were measured in cation and anion modes by capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF/MS) system (Agilent Technologies Inc., Santa Clara, CA, USA) [29 (link),30 (link),49 (link)]. Peaks were extracted using automatic integration software (MasterHands ver. 2.13.0.8.h; Keio University, Tokyo, Japan). The CE system was equipped with an Agilent 6210 TOF mass spectrometer, Agilent G1603A CE-MS Adapter Kit, Agilent 1100 Isocratic HPLC Pump, and Agilent G1607A CE-ESI-MS Sprayer Kit (Agilent Technologies, Waldbronn, Germany). The system was controlled by Agilent G2201AA ChemStation software version B.03.01 for CE (Agilent Technologies Inc., Santa Clara, CA, USA). A fused silica capillary column with 50 µm i.d × 80 cm total length (Agilent Technologies, Waldbronn, Germany) was used for separation. Analytical conditions were set as follows: run buffer (cation buffer solution (p/n: H3301–1001) and anion run buffer (p/n: I3302–1023), which were also used for rinsing; MS ionization was conducted in positive and negative ion modes; sample injection pressure was 50 mbar for 10 s; CE voltage was 27 kV (cation) and 30 kV (anion); MS capillary voltage was 4000 V (cation) and 3500 V (anion); and the MS scan range was m/z 50–1000.
Agilent g2201aa chemstation software version b 03.01 for ce
Manufactured by Agilent Technologies
Sourced in United States, Germany
The Agilent G2201AA ChemStation software version B.03.01 is a laboratory software package designed for data acquisition, analysis, and reporting for capillary electrophoresis (CE) applications. It provides a comprehensive platform for managing CE-based analytical workflows.
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Lab products found in correlation
Agilent g1607a ce esi ms sprayer kit, by Agilent Technologies (2 mentions)
Agilent g1603a ce ms adapter kit, by Agilent Technologies (2 mentions)
Agilent 1100 isocratic hplc pump, by Agilent Technologies (2 mentions)
Agilent 6210 tof mass spectrometer, by Agilent Technologies (1 mentions)
Ce tofms system, by Agilent Technologies (1 mentions)
Fused silica capillary column, by Agilent Technologies (1 mentions)
Ce capillary electrophoresis system, by Agilent Technologies (1 mentions)
Agilent 6210 time of flight mass spectrometer, by Agilent Technologies (1 mentions)
2 protocols using agilent g2201aa chemstation software version b 03.01 for ce
1
Metabolite Profiling by CE-TOF/MS
2
Intracellular Metabolite Profiling of Activated T Cells
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CD3+ T cells (around 1.8 × 107 cells per sample) were activated for 36 hours in conditional media (RPMI-1640) containing 2 mM 13C5-glutamine, 1.15 mM 13C6-arginine, or 0.17 mM 13C5-proline, and then used for the extraction of intracellular metabolites as described above. Metabolome measurements were carried out through a facility service at Human Metabolome Technologies Inc. (Tsuruoka, Japan). CE-TOFMS measurement was carried out using an Agilent CE Capillary Electrophoresis System equipped with an Agilent 6210 Time of Flight mass spectrometer, Agilent 1100 isocratic HPLC pump, Agilent G1603A CE-MS adapter kit, and Agilent G1607A CE-ESI-MS sprayer kit (Agilent Technologies, Waldbronn, Germany). The systems were controlled by Agilent G2201AA ChemStation software version B.03.01 for CE (Agilent Technologies, Waldbronn, Germany). The metabolites were analyzed by using a fused silica capillary [50 μm internal diameter (i.d.)× 80 cm total length], with commercial electrophoresis buffer (solution ID: H3301-1001 for cation analysis and H3302-1021 for anion analysis, Human Metabolome Technologies) as the electrolyte. The sample was injected at a pressure of 50 mbar for 10 s (approximately 10 nl) in the cation analysis and 25 s (approximately 25 nl) in the anion analysis. The spectrometer was scanned from m/z 50 to 1000.
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