Anti pan actin antibody

Cycloheximide, 3-NP (3-nitropropionic acid), etoposide and protease inhibitor cocktail were purchased from Sigma–Aldrich. MG132 was from Tocris Bioscience. Antibodies against c-Myc, FLAG, GFP, HA (haemagglutinin), p53, ubiquitin and HSP60 (heat-shock protein 60) were from Santa Cruz Biotechnology, and anti-Drp1 (DLP1) was from BD Biosciences. Anti-PARP [poly(ADP-ribose) polymerase] antibody was purchased from Cell Signaling Technology. Anti-VDAC (voltage-dependent anion channel) and anti-(cytochrome c) antibodies were from MitoScience, and anti-pan-actin antibody was from Sigma–Aldrich. Anti-Bax and anti-HMGB1 (high-mobility group box B1) antibodies were from Proteintech. Anti-(mouse IgG) and horseradish-peroxidase-linked species-specific antibodies were from Thermo Scientific and Abcam (only recognizing the light chain).

Cycloheximide (CHX) and the protease inhibitor cocktail were purchased from Sigma–Aldrich. Proteasome inhibitor MG132 and VCP inhibitor DBeQ were from Tocris Bioscience. Antibodies against c-Myc (sc-40, 1:1000), GFP (sc-9996, 1:1000), HA (haemagglutinin, sc-7392, 1:1000), ubiquitin (sc-271289, 1:1000), HSP60 (sc-13115, 1:1000), MCL1 (sc-819, 1:1000), NPL4 (sc-134746, 1:1000), and p47 (sc-376614, 1:1000) were from Santa Cruz Biotechnology. VDAC (ab14734, 1:2000), VCP (ab109240, 1: 10,000), and UBXD1 (ab103651, 1:1000) antibodies were from Abcam. Anti-pan-actin antibody (A1978, 1: 10,000) was from Sigma–Aldrich. Anti-MFN1 (H00055669-M04, 1:1000) and MFN2 (H00009927-M01, 1:1000) antibodies were from Abnova and anti-UFD1L (NBP1-03339, 1:1000) was from Novus. Anti-mouse and Anti-rabbit IgG antibodies were from Thermo Scientific.