Siglec g

Manufactured by Thermo Fisher Scientific

Siglec-G is a laboratory equipment product offered by Thermo Fisher Scientific. It is a receptor protein that plays a role in the regulation of the immune response. The core function of Siglec-G is to bind to specific carbohydrate structures, which can influence cellular signaling and immune cell activation. This product is intended for use in research applications.

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Lab products found in correlation

Siglec e, by Thermo Fisher Scientific (2 mentions) Taqman probe, by Thermo Fisher Scientific (2 mentions) Genejet rna purification kit, by Thermo Fisher Scientific (1 mentions) Siglecf, by Thermo Fisher Scientific (1 mentions) Superscript vilo, by Thermo Fisher Scientific (1 mentions) Puromycin, by Merck Group (1 mentions)

2 protocols using siglec g

Quantitative Analysis of Siglec Expression

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Quantitative reverse-transcription PCR was used to determine relative mRNA levels in macrophages. Where stated, BMDM were stimulated with LPS at 1 μg/mL overnight. RNA extraction was done using a GeneJET RNA Purification Kit (ThermoFisher Scientific). cDNA was made using SuperScript™ VILO™ (ThermoFisher Scientific) reverse transcriptase. Taq-man probes used for CD22, CD33, Siglec-E, Siglec-F, Siglec-G, and Abt1 were from Thermo Scientific.

Imbert P.R., Saric A., Pedram K., Bertozzi C.R., Grinstein S, & Freeman S.A. (2020). An acquired and endogenous glycocalyx forms a bidirectional ‘don’t eat’ and ‘don’t eat me’ barrier to phagocytosis. Current biology : CB, 31(1), 77-89.e5.

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Generating Stable Clones with Silenced Neu1, Siglec-e or Siglec-g

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D2SC/1 dendritic cells were obtained from Dr. Peter G. Stock and Sang-Mo Kang (University of California, San Francisco, CA) with the permission from Dr. Paola Ricciardi-Castagnoli (University of Perugia, Italy), and maintained in Dulbeco’s minimal essential medium supplemented with 10% heat-inactivated fetal calf serum and 1% penicillin and streptomycin. The lentiviral vectors expressing either Neu1, Siglec-e or Siglec-g shRNAs were from ThermoFisher Scientific. Puromycin was purchased from Sigma. Stable clones were obtained after selection with Puromycin (2.5 µg/ml) for 2 weeks after infection.

Wu Y., Ren D, & Chen G.Y. (2016). Siglec-E negatively regulates the activation of Toll-like Receptor 4 by controlling its endocytosis. Journal of immunology (Baltimore, Md. : 1950), 197(8), 3336-3347.

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