Disodium hydrogen phosphate

Chlorogenic acid, MPTP, and normal goat serum (NGS) were bought from Sigma–Aldrich (St. Louis, MO, United States). Acetic acid, ammonium chloride, bovine serum albumin (BSA), potassium chloride, disodium hydrogen phosphate, reduced nicotinamide adenine dinucleotide phosphate (NADPH), and sodium dihydrogen phosphate were procured from Sisco Research Laboratories (SRL, Mumbai, India). Protein estimation kit by Bradford GeNeiTM, hydrogen peroxide (H₂O₂), and potassium dichromate were purchased from Merck (Darmstadt, Germany), and sodium dodecyl sulphate (SDS), thiobarbituric acid (TBA), DABCO, and Griess reagent were obtained from HiMedia (Mumbai, India). Paraformaldehyde and sodium nitrite were bought from Lobachemie, India. Primary antibodies for TH (SC-25269), iNOS (SC-651), and glial fibrillary acidic protein (GFAP; SC-33673) were purchased from Santa Cruz, Biotechnology (Santa Cruz, CA, United States), and the primary antibodies for TNF-α (ab1793) and NF-κB (ab16502) were obtained from Abcam Life Science, Biogenuix Medsystems Pvt. Ltd. (New Delhi, India), and secondary fluorescent-tagged antibodies such as Cy2 conjugated and Cy3 conjugated for immunohistochemistry were bought from Merck Millipore and Chemicon, respectively.

Raloxifene HCl (RLX), USP 99.7% purity, batch number CFR018007 was purchased from BDR Pharmaceuticals International (Mumbai, India). Cremophor^® RH40 (polyoxyl 40 hydrogenated castor oil) was kindly provided by BASF SE (Ludwigshafen, Germany). Transcutol^® HP was a kind gift from Gattefosse (Lyon, France). Miglyol^® 812 (caprylic/capric triglyceride) was a kind gift from EIPICO for pharmaceutical industries (Cairo, Egypt). Hydroxypropyl methyl cellulose (HPMC), methocel™ K100LV (100 cP at a 2% addition rate in water), was purchased from Chempoint (Bellevue, WA, USA). Poly vinyl alcohol (PVA) (average Mw 146,000–186,000), chitosan (CS) high molecular weight (800–2000 cP), and acetonitrile were purchased from Sigma–Aldrich, Co. (St. Louis, MO, USA). Normal saline was purchased from Otsuka Pharmaceutical Co., S.A.E (Cairo, Egypt). Xylazine (XYLA-JECT^®) was purchased from ADWIA Pharmaceuticals Co. (Cairo, Egypt). Ethyl acetate and formic acid were bought from El-Nasr Pharmaceutical Chemicals Co. (Cairo, Egypt). Olmesartan obtained from AstraZeneca (Cairo, Egypt). Potassium dihydrogen phosphate and disodium hydrogen phosphate were purchased from Sisco Research Laboratories (Mumbai, India). HPLC-grade methanol was purchased from Research-lab Fine Chem Industries (Mumbai, India). All other chemicals and solvents were of analytical grade and used as received.

Acetic acid, disodium hydrogen phosphate, nicotinamide adenine dinucleotide reduced form (NADH), potassium chloride, phenazinemethosulphate, nitro-blue tetrazolium, disodium phosphate and sodium dihydrogen phosphates were procured from Sisco Research Laboratories (Mumbai, India). Reagents for Bradford assay were purchased from GeNei (Bengaluru, India). Hydrogen peroxide and potassium-dichromate were purchased from Merck (Darmstadt, Germany). Sodium dodecyl sulphate (SDS), thiobarbituric acid (TBA), trireagent, and zinc sulphate were procured from Sigma-Aldrich (St. Louis, MO, USA). Rabbit polyclonal to NMDAR2A (ab16646) antibody were procured from Abcam (place), mouse monoclonal anti-β-actin (sc47778) purchased from Santacruz (USA), biotinylated Goat polyclonal to Rabbit IgG and anti-mouse secondary antibodies, anti-goat AP conjugated and bovine anti-rabbit (AP) conjugated secondary antibodies were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). DABCO, anti-fading agent was purchased from Fluka analytical (Shanghai, China). Secondary antibody, GT × Rb IgG-Biotin conjugated (62111028001A) and GT × Ms IgG Biotin-conjugated (cat#105261) (GeNei, Bengaluru, India). Streptavidin-peroxidase, normal goat serum and DAB system was procured from Genei Pvt. India Ltd. Polyvinylidene difloride (PVDF) membranes were procured from Merck Millipore (USA).

Etodolac was kindly gifted from European Egyptian Pharmaceutical Industries, Egypt and manufactured by Polpharma Pharmaceutical Works, Warsaw, Poland. Poloxamer 407 (Plx 407; Pluronic acid F127), phosphotungstic acid and MO were obtained from Sigma–Aldrich Chemie GmbH, Germany. Dialysis tubing cellulose membrane (molecular weight cutoff 12,000 g/mole) was purchased from Sigma Chemical Company (Sigma-Aldrich Corp., St. Louis, MO, USA). Poly(vinyl alcohol) (PVA; M.Wt. 22000 Da) was purchased from Sigma-Aldrich (Sigma-Aldrich Co., St. Louis, MO, USA). Potassium dihydrogen phosphate and disodium hydrogen phosphate were purchased from Sisco Research Laboratories Pvt. Ltd., Mumbai, India. HPLC grade of acetonitrile and methyl-t-butyl-ether were purchased from SDFCL – S. D. Fine Chemical Limited, Mumbai, India.