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2 protocols using dihydroceramide 1 phosphate

1

Comprehensive Lipid Standard Preparation

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Lipid standards [MG (15:1), diacylglycerols (8:0-8:0), TG (11:1-11:1-11:1) chE (10:0)] were purchased from Larodan Fine Chemicals AB (MI, USA). Other lipids [Cer (d18:1-12:0), dCer (d18:0-12:0), C1P (d18:1-12:0), dihydroceramide-1-phosphate (d18:0-16:0), SO (17:1), sphinganine; SA (17:0), SO-1-phosphate (17:1), sphinganine-1-phosphate (17:0), SM (d18:1-12:0), dSM (d18:1-12:0), PC (10:1-10:1), LPC (13:0), PE (10:1-10:1), LPE (14:0), PG (10:1-10:1), LPG (14:0), phosphatidic acid (10:1-10:1), lysophosphatidic acid; LPA (17:0), PS (10:1-10:1), lysophosphatidylserine (17:1), phosphatidylinositol (16:0), lysophosphatidylinositol (13:0)] were purchased from Avanti Polar Lipids, Inc (AL, USA). ChE was dissolved in chloroform and all other lipid standards were dissolved in methanol, stored at –20° C, and diluted to 1 μg/mL for use in lipid extraction of the sample.
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2

Comprehensive Lipid Standards Acquisition

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HPLC-grade methanol, acetonitrile, water, chloroform, and isopropyl alcohol were purchased from J.T. Baker (Phillipsburg, NJ, USA). HPLC-grade formic acid, hydrochloric acid, ammonium formate, and acetic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipid standards used in this study were purchased from Larodan Fine Chemicals AB [Cholesteryl ester; CE (10:0), Monoacylglycerols; MG (15:1), Diacylglycerols; DG (8:0-8:0), Triacylglycerols; TG (11:1-11:1-11:1)] and Avanti Polar Lipids, Inc. [Phosphatidylcholine; PC (10:1-10:1), LysoPhosphatidylcholine; LPC (13:0), Phosphatidylethanolamine; PE (10:1-10:1), Lysophosphatidylethanolamine; LPE (14:0), Phosphatidylglycerol; PG (10:1-10:1), Lysophosphatidylglycerol; LPG (14:0), Phosphatidic acid; PA (10:1-10:1), LysoPhosphatidic acid; LPA (17:0), Phosphatidylserine; PS (10:1-10:1), Lysophosphatidylserine; LPS (17:1), Phosphatidylinositol; PI (16:0), Lysophosphatidylinositol; LPI (13:0), Ceramide; Cer (d18:1-12:0), Dihydroceramide; dCer (d18:0-12:0), Ceramide-1-phosphate; Cer1P (d18:1-12:0), Dihydroceramide-1-phosphate; dCer1P (d18:0-16:0), Sphingosine; SO (17:1), Sphinganine; SA (17:0), Sphingosine-1-phosphate; SO1P (17:1), Sphinganine-1-phosphate; SA1P (17:0), Sphingomyelin; SM (d18:1-12:0), Dihydrosphingomyelin; dSM (d18:1-12:0)].
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