The normal human breast epithelial cell line (MCF-10A), the 293 cell line and four TNBC cell lines (HCC1143, MDA-MB-231, BT-549 and MDA-MB-453) were acquired from American Type Culture Collection. Cells were incubated in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (FBS), and 1% penicillin and streptomycin (all from Thermo Fisher Scientific, Inc.). The cells were fostered at 37°C in an atmosphere containing 5% CO2 and the medium was changed every 2–3 days.
293 cell line
Manufactured by American Type Culture Collection
Sourced in United States
The 293 cell line is a widely used human embryonic kidney cell line. It was originally derived from human embryonic kidney cells transformed with sheared adenovirus 5 DNA. The 293 cell line is known for its ability to efficiently express recombinant proteins and is commonly used in cell biology research, protein production, and gene expression studies.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 231, by American Type Culture Collection (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Bt549, by American Type Culture Collection (1 mentions)
Hcc1143, by American Type Culture Collection (1 mentions)
Mda mb 453, by American Type Culture Collection (1 mentions)
Umuc3, by American Type Culture Collection (1 mentions)
Sv huc 1 cells, by American Type Culture Collection (1 mentions)
Sw780, by American Type Culture Collection (1 mentions)
Ht1376, by American Type Culture Collection (1 mentions)
Rnazol rt rna isolation reagent, by Molecular Research Center (1 mentions)
Lncap cell line, by American Type Culture Collection (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
96 well plate, by Sarstedt (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
Mda mb 468, by Thermo Fisher Scientific (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Hyclone, by GE Healthcare (1 mentions)
6 protocols using 293 cell line
1
Culturing Breast and Kidney Cell Lines
2
Isolation and Characterization of Healthy Human T Cells
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Peripheral blood T cells were isolated from the whole blood samples of healthy donors (2 males and 2 females, aged between 23 and 33, with normal blood routine, normal liver and kidney function, and no serious diseases such as malignant tumors) using Ficoll-Hypaque density-gradient centrifugation (20 min, 24°C, 800 × g). Written informed consent was obtained from all donors. The study procedures were approved by The Local Ethics Committee of Guangxi Medical University (Nanning, China). The 293-cell line was purchased from American Type Culture Collection (Manassas, VA, USA).
A total of 60 female C57BL/6 mice, aged 4–6 weeks, between 13 and 18 g, were purchased from Guangxi Laboratory Animal Center (Guangxi, China) and raised in laminar flow cabinets in a specific pathogen-free environment (temperature, 23±1°C; humidity, 50±10%; 12-h light/dark cycle starting at 7:00 a.m.; with free access to food and water). All protocols were approved by the Animal Ethics Committee of Guangxi Medical University (Guangxi, China).
A total of 60 female C57BL/6 mice, aged 4–6 weeks, between 13 and 18 g, were purchased from Guangxi Laboratory Animal Center (Guangxi, China) and raised in laminar flow cabinets in a specific pathogen-free environment (temperature, 23±1°C; humidity, 50±10%; 12-h light/dark cycle starting at 7:00 a.m.; with free access to food and water). All protocols were approved by the Animal Ethics Committee of Guangxi Medical University (Guangxi, China).
3
Cultivating Bladder Cell Lines
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Human BCa T24, UM-UC-3, SW780, HT1376, RT4 and J82 cell lines, immortalized human bladder epithelium SV-HUC-1 cells and the 293 cell line were purchased from the American Type Culture Collection (Manassas, VA, USA). The cells were cultured according to the manufacturer's instructions at 37°C in an atmosphere of 5% CO2. Total mRNA and miRNA were independently extracted from the cells using RNAzol RT RNA Isolation Reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and stored at −20°C for subsequent analysis.
4
Cell Line Characterization and Reagents
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The 293 cell line, human normal prostate epithelial RWPE-1 cell line, and human Pca DU-145, PC-3 and LNCaP cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). FH55 [F5682; high performance liquid chromatography (HPLC) purity ≥98%] and LiCl (746460; HPLC ≥99%) were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany).
5
TNBC Cell Line Treatment with Cisplatin
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The human TNBC MDA-MB-231 and MDA-MB-468 cell lines, and 293 cell line were purchased from American Type Culture Collection (Manassas, VA, USA). The 293, MDA-MB-231 and MDA-MB-468 cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; HyClone; GE Healthcare Life Sciences, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, Inc.) in an incubator with 5% CO2 at 37°C.
Cisplatin was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). For Cisplatin treatment, MDA-MB-231 and MDA-MB-468 cells (3×103 cells/well) were seeded into 96-well plates (Sarstedt, Nümbrecht, Germany) and incubated with 25 μM Cisplatin in DMEM in an incubator with 5% CO2 at 37°C for 24 h, and then subjected to subsequent analyses.
Cisplatin was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). For Cisplatin treatment, MDA-MB-231 and MDA-MB-468 cells (3×103 cells/well) were seeded into 96-well plates (Sarstedt, Nümbrecht, Germany) and incubated with 25 μM Cisplatin in DMEM in an incubator with 5% CO2 at 37°C for 24 h, and then subjected to subsequent analyses.
6
Prostate Cancer Cell Lines and Tissue Samples
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The human prostate cancer cell lines PC-3, DU145, CL1, LNCaP and C4-2B were obtained from the American Type Culture Collection. The cell lines were maintained in RPMI-1640 medium (Life Technologies; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal calf serum (FCS; Life Technologies; Thermo Fisher Scientific, Inc.) and 100 µg/ml penicillin and 100 µg/ml streptomycin. The 293 cell line was obtained from the American Type Culture Collection and maintained in DMEM (Life Technologies; Thermo Fisher Scientific, Inc.) with 10% FCS. Cells were cultured at 37°C with 5% CO2. Benign prostatic hyperplasia (BPH) tissue (n=3; patient age 65–77) and prostate cancer tissue samples (n=3; patient age 72–81; Gleason score 7–9) were collected from March to May 2018 by transurethral resection of the prostate. All tissue samples were collected in the West China Hospital according to the ethical guidelines and procedures approved by the institutional supervisory committee. Informed consent was obtained according to the institutional guidelines. Fresh tissue samples were stored immediately at −80°C.
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