Secondary hrp conjugated antibodies

2-Methoxystypandrone was isolated from the root of P. cuspidatum Sieb, as reported previously;(21 (link)) DTT and MTT were purchased from Genebase (Shanghai, China); GSH was obtained from Shanghai Sibas Bioscience (Shanghai, China); IL-6 and IFN-α were from Peprotech (Saint Paul, MN, USA); TNF-α was from R&D Systems (Minneapolis, MN, USA); IFN-γ was obtained from Shanghai Clone (Shanghai, China); anti-phosphorylated STAT3 (Tyr705), anti-phophorylated JAK2 (Tyr1007/1008), anti-JAK2, anti-phophorylated JAK1 (Tyr1022/1023), anti-JAK1, anti-phophorylated TYK2(Tyr1054/1055), anti-TYK2, anti-phosphorylated IκB-α (Ser32/36), anti-IκB-α, anti-phosphorylated IKK-αβ (Ser176/180 for IKKα and Ser177/181 for IKKβ) and anti-IKK-α antibodies were obtained from Cell Signaling Technology (Boston, MA, USA); anti-STAT3, anti-GP130 and anti-α-Tubulin antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA); anti-β-Actin antibody was from Abmart (Shanghai, China); anti-GAPDH antibody was obtained from Kang Chen Bioscience (Shanghai, China); secondary HRP-conjugated antibodies were from Multi Sciences Biotech (Hangzhou, China); and the Apoptosis Detection Kit was from Shanghai MaiYueEr Bioscience (Shanghai, China).