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Anti epcam magnetic beads

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Anti-EpCAM magnetic beads are a type of laboratory equipment used for the isolation and enrichment of epithelial cells. They consist of magnetic particles coated with antibodies that specifically bind to the EpCAM (Epithelial Cell Adhesion Molecule) protein expressed on the surface of epithelial cells. These beads can be used to separate and concentrate epithelial cells from a mixed cell population, facilitating further analysis or processing.

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Lab products found in correlation

Pharm lyse solution, by BD (2 mentions) Anti cd45 fitc, by Thermo Fisher Scientific (2 mentions) Anti epcam pe, by BD (2 mentions) Magsweeper16, by Illumina (2 mentions)

Close spelling variants of this product

Magnetic beads (6 citations)

2 protocols using anti epcam magnetic beads

1

Circulating Tumor Cell Isolation Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For each 3.75 mL of blood, red blood cell (RBC) lysis was first performed using 1× Pharm Lyse solution (BD Biosciences). The RBC-depleted sample was then incubated with 4 tests of FITC-anti-CD45 (eBiosciences; clone HI30) for 30 min at 4 °C, followed by incubation with anti- EpCAM magnetic beads (Illumina)16 (link) for 30 min at 4 °C. PE-anti-EpCAM (BD Biosciences; clone EBA-1) was then added for 30 min at 4 °C prior to immunomagnetic isolation using the Illumina MagSweeper16 (link).
Lohr J.G., Adalsteinsson V.A., Cibulskis K., Choudhury A.D., Rosenberg M., Cruz-Gordillo P., Francis J., Zhang C.Z., Shalek A.K., Satija R., Trombetta J.T., Lu D., Tallapragada N., Tahirova N., Kim S., Blumenstiel B., Sougnez C., Lowe A., Wong B., Auclair D., Van Allen E.M., Nakabayashi M., Lis R.T., Lee G.S., Li T., Chabot M.S., Ly A., Taplin M.E., Clancy T.E., Loda M., Regev A., Meyerson M., Hahn W.C., Kantoff P.W., Golub T.R., Getz G., Boehm J.S, & Love J.C. (2014). Whole exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer. Nature biotechnology, 32(5), 479-484.
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2

Circulating Tumor Cell Isolation Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For each 3.75 mL of blood, red blood cell (RBC) lysis was first performed using 1× Pharm Lyse solution (BD Biosciences). The RBC-depleted sample was then incubated with 4 tests of FITC-anti-CD45 (eBiosciences; clone HI30) for 30 min at 4 °C, followed by incubation with anti- EpCAM magnetic beads (Illumina)16 (link) for 30 min at 4 °C. PE-anti-EpCAM (BD Biosciences; clone EBA-1) was then added for 30 min at 4 °C prior to immunomagnetic isolation using the Illumina MagSweeper16 (link).
Lohr J.G., Adalsteinsson V.A., Cibulskis K., Choudhury A.D., Rosenberg M., Cruz-Gordillo P., Francis J., Zhang C.Z., Shalek A.K., Satija R., Trombetta J.T., Lu D., Tallapragada N., Tahirova N., Kim S., Blumenstiel B., Sougnez C., Lowe A., Wong B., Auclair D., Van Allen E.M., Nakabayashi M., Lis R.T., Lee G.S., Li T., Chabot M.S., Ly A., Taplin M.E., Clancy T.E., Loda M., Regev A., Meyerson M., Hahn W.C., Kantoff P.W., Golub T.R., Getz G., Boehm J.S, & Love J.C. (2014). Whole exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer. Nature biotechnology, 32(5), 479-484.
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