Integrin β1

Manufactured by R&D Systems

Sourced in Germany

Integrin β1 is a cell surface receptor that mediates cell-cell and cell-extracellular matrix interactions. It is a subunit of the Integrin family of proteins and is involved in various cellular processes.

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Lab products found in correlation

Integrin β1, by Abcam (2 mentions) Total integrin β1, by BD (2 mentions) Active integrin β1, by BD (2 mentions) Smad2 3, by Cell Signaling Technology (2 mentions) Pdgfrβ 28e1, by Cell Signaling Technology (2 mentions) Periostin, by Santa Cruz Biotechnology (2 mentions) Sprr3, by Proteintech (2 mentions) P pdgfrβ tyr751, by Cell Signaling Technology (2 mentions) P smad2, by Cell Signaling Technology (2 mentions) P fak, by Cell Signaling Technology (2 mentions) Gapdh, by Merck Group (2 mentions) Phospho akt, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions) Alpha smooth muscle actin α sma, by Merck Group (2 mentions) Cxcr4, by R&D Systems (2 mentions) Antibodies to β actin, by Merck Group (2 mentions) Secondary rhodamine coupled antibodies for immunofluorescence and anti ki67, by Dianova (2 mentions) Alkaline phosphatase linked antibodies for western blotting, by Merck Group (2 mentions) Tnf β, by Thermo Fisher Scientific (2 mentions) Tnf β, by Gene Tech (2 mentions)

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Anti-β1 integrin (4 citations)

6 protocols using integrin β1

Integrin and Signaling Pathway Analysis

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The following antibodies were used: Alpha Smooth Muscle Actin (α-SMA) (1:1000; Sigma A2547), Periostin (1:100; Santa Cruz, SC67233), collagen I (1:500; MDBioproducts, #203002A), phospho-Akt (1:500; Cell Signaling, #4058S), Akt (1:500; Cell Signaling, #9272), pSmad2 (1:500; Cell Signaling, #3108S), Smad2/3 (1:500; Cell Signaling, #5678S), β-actin (1:5000; Sigma, #A5441) and SPRR3 (1:500; Proteintech group 11742–1-AP, or custom made) p-p38 (1:500; Cell Signaling), p38 (1:500; Cell Signaling), FAK (1:1000; Cell Signaling, #3285), p-FAK (1:500; Cell Signaling; #3283), GAPDH (1:500; Millipore; MAB374), p-PDGFRβ (Tyr751) (1:1000; Cell Signaling; #3161), PDGFRβ (28E1) (1:1000; Cell Signaling; #3169), integrin β1 (1:1000; R&D; #AF2405), total integrin β1 (flow cytometry, BD Pharmingen #550530), active integrin β1 (BD Pharmingen; #553715) and integrin β1 (Abcam; ab24693; 10 ug/ml for proliferation).

Saraswati S., Lietman C.D., Li B., Mathew S., Zent R, & Young P.P. (2020). Small proline‐rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts. The FASEB Journal, 34(6), 7885-7904.

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Comprehensive Antibody Analysis for Cell Signaling

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The following antibodies were used: Alpha Smooth Muscle Actin (α‐SMA) (1:1000; Sigma A2547), Periostin (1:100; Santa Cruz, SC67233), collagen I (1:500; MD Bioproducts, #203002A), phospho‐Akt (1:500; Cell Signaling, #4058S), Akt (1:500; Cell Signaling, #9272), pSmad2 (1:500; Cell Signaling, #3108S), Smad2/3 (1:500; Cell Signaling, #5678S), β‐actin (1:5000; Sigma, #A5441), and SPRR3 (1:500; Proteintech group 11742‐1‐AP, or custom made) p‐p38 (1:500; Cell Signaling), p38 (1:500; Cell Signaling), FAK (1:1000; Cell Signaling, #3285), p‐FAK (1:500; Cell Signaling; #3283), GAPDH (1:500; Millipore; MAB374), p‐PDGFRβ (Tyr751) (1:1000; Cell Signaling; #3161), PDGFRβ (28E1) (1:1000; Cell Signaling; #3169), integrin β1 (1:1000; R&D; #AF2405), total integrin β1 (flow cytometry, BD Pharmingen #550530), active integrin β1 (BD Pharmingen; #553715), and integrin β1 (Abcam; ab24693; 10 ug/ml for proliferation).

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Comprehensive Protein Expression Analysis

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Cells were lysed by RIPA buffer [150 mM NaCl, 1% NP40, 0.25% deoxycholate and 10 mM Hepes (pH 7.4)] containing a protease inhibitor cocktail (Roche Molecular Biochemicals). Protein concentrations were quantified using the bicinchoninic acid (BCA) method (Pierce). Thirty micrograms of protein per sample was boiled in loading buffer and electrophoresed in 8–12% gradient SDS polyacrylamide gels (Invitrogen) and transferred onto nitrocellulose membranes. The membranes were probed with antibodies (CDH17, Integrin β1, Integrin β4, Integrin β5, p-p53, p53, p21 were from R&D, Raf, p-MEK, MEK, p-ERK, ERK were from Cell signaling, and K-Ras was from Santa Cruz) and followed by a peroxidase-conjugated immunoglobulin. Western blots were visualized with an enhanced chemiluminescence (ECL) kit (GE, Healthcare).

Lin Z., Zhang C., Zhang M., Xu D., Fang Y., Zhou Z., Chen X., Qin N, & Zhang X. (2014). Targeting Cadherin-17 Inactivates Ras/Raf/MEK/ERK Signaling and Inhibits Cell Proliferation in Gastric Cancer. PLoS ONE, 9(1), e85296.

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Integrin Signaling Modulation in Myeloma

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MSCs were cultured alone or co-cultured with myeloma cells with or without G3335 or neutralizing antibodies for 48 hours. In some experiments, MG132 was added to the cultures 6 hours prior to the cell collection. Addition of DMSO served as vehicle control for inhibitor experiment; addition of IgG served as neutralizing antibody control.
Quantitative real-time PCR was performed as described (46 (link)). The primers are listed in table S9. For Western blotting, cells were lysed with 1× lysis buffer (Cell Signaling Technology), subjected to 4–20% gradient gel electrophoresis, transferred to, and immunoblotted with antibodies against integrin α4 (R&D Systems), integrin β1, VCAM1, PKC, MURF1, and phosphorylated isoforms of PKC along with p-PKC-pan (Cell Signaling Technology) and PPARɣ2 (Santa Cruz Biotechnology). The membrane was stripped and re-probed with an antibody against β-actin to ensure equal protein loading, and finally signals was detected using peroxidase-conjugated secondary antibody followed by enhanced chemiluminescence system (Millipore) in the MiniChem system (Saizhi Biotech), and quantitative analysis of blots were performed using the Fiji based ImageJ software (version 1.51n, National Institute Health, Bethesda, MA, USA).

Liu Z., Liu H., He J., Lin P., Tong Q, & Yang J. (2020). Myeloma cells shift osteoblastogenesis to adipogenesis by inhibiting the ubiquitin ligase MURF1 in mesenchymal stem cells. Science signaling, 13(633), eaay8203.

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Monoclonal Antibodies and Cellular Targets in Cancer Research

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Monoclonal antibodies to p65, and phospho-specific p65-NF-κB, MMP-9, CXCR4, cleaved-Caspase-3, and β1-Integrin, were obtained from R&D Systems (Heidelberg, Germany). Antibodies to β-actin were from Sigma-Aldrich (Taufkirchen, Germany). Secondary rhodamine-coupled antibodies for immunofluorescence and anti-Ki67 were from Dianova (Hamburg, Germany), and alkaline phosphatase-linked antibodies for Western blotting were from EMD Millipore (Schwalbach, Germany). Monoclonal anti-ALDH1 was obtained from Acris Antibodies GmbH (Herold, Germany). Monoclonal anti-CD133 and anti-CD44 were purchased from Abcam PLC (Cambridge, UK). MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), 4′,6-diamidino-2-phenylindole (DAPI), and alginate were from Sigma-Aldrich (Taufkirchen, Germany). TNF-β was purchased from eBiosciences (Frankfurt, Germany). Furthermore, TNF-β was given as a kind gift by Genetech (South San Francisco, CA, USA). Calebin A was a generous gift from Sabinsa Corporation (East Windsor, NJ, USA). Calebin A was diluted as 10,000 µM stock solution in dimethyl sulfoxide (DMSO) and further diluted in cell culture medium for experimental investigations. The final concentration of DMSO did not exceed 0.1% during the experiments.

Buhrmann C., Kunnumakkara A.B., Kumar A., Samec M., Kubatka P., Aggarwal B.B, & Shakibaei M. (2021). Multitargeting Effects of Calebin A on Malignancy of CRC Cells in Multicellular Tumor Microenvironment. Frontiers in Oncology, 11, 650603.

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Molecular Markers Analysis in Cancer

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Monoclonal antibodies to p65, as well as phospho-specific p65-NF-κB, MMP-9, CXCR4, cleaved-Caspase-3, β1-Integrin and PARP, were obtained from R&D Systems (Heidelberg, Germany). Antibodies to β-Actin were from Sigma-Aldrich (Taufkirchen, Germany). Secondary rhodamine-coupled antibodies for immunofluorescence and anti-Ki67 were from Dianova (Hamburg, Germany) and alkaline phosphatase-linked antibodies for Western blotting from EMD Millipore (Schwalbach, Germany). MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DAPI, 5-Fluorouracil (5-FU), alginate, BMS-345541 and dithiothreitol (DTT) were from Sigma-Aldrich (Taufkirchen, Germany). Stock solutions of BMS-345541 (1000 µM) and of DTT (1000 mM) were prepared in PBS and further diluted in normal cell culture growth medium to obtain final concentrations. Then, 5-FU was diluted as a 1000 µM stock solution in ethanol and further diluted in the cell culture medium. Final concentrations of ethanol did not exceed 0.1% during treatment. TNF-β was purchased from eBiosciences (Frankfurt, Germany). Further, TNF-β was given as a kind gift by Genetech (South San Francisco, CA, USA). Calebin A (CA), with a purity of 99.65%, was a generous gift from Sabinsa Corporation (East Windsor, NJ, USA). Epon for electron microscopy was purchased from Plano (Marburg, Germany).

Buhrmann C., Kunnumakkara A.B., Popper B., Majeed M., Aggarwal B.B, & Shakibaei M. (2020). Calebin A Potentiates the Effect of 5-FU and TNF-β (Lymphotoxin α) against Human Colorectal Cancer Cells: Potential Role of NF-κB. International Journal of Molecular Sciences, 21(7), 2393.

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