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Af micro nikkor 60 mm 1 2.8 d lens

Manufactured by Nikon
Sourced in United States

The AF Micro Nikkor 60mm f/2.8D is a mid-range macro lens designed for Nikon DSLR cameras. It offers a 1:1 maximum reproduction ratio, allowing for close-up photography. The lens features an autofocus motor and a minimum focusing distance of 0.219m.

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3 protocols using af micro nikkor 60 mm 1 2.8 d lens

1

Photographic Capture of Plant Tissues

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Photographs of the plants and their tissues were recorded using a Nikon D7000 digital camera with an AF Micro Nikkor 60 mm 1:2.8 D lens or AF-S Nikkor 18–70 mm DX lens (Nikon Inc., Melville, NY, United States) under tungsten lamps (Philips, 120 V, 300 W). The camera was set manually for all parameters including ISO sensitivity, focus, f-stop and time. A photography gray card was used as a reference to get the correct exposure. The callus images in petri plates were observed and photographed in a Leica MZ16-F (Leica Microsystems, Inc., Buffalo Grove, IL, United States) stereo zoom light microscope equipped with a QImaging Retiga 2000 R fast cooled, digital color camera.
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2

Detailed Imaging Protocol for Plant Specimens

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The photographs of the plants were recorded using a Nikon D7000 digital camera with an AF Micro Nikkor 60 mm 1:2.8 D lens or AF-S Nikkor 18–70 mm DX lens (Nikon Inc., Melville, NY) under tungsten lamps (Philips, 120 V, 300 W). The camera was set manually for all parameters including ISO sensitivity, focus, f-stop and time. A photography gray card was used as a reference to get the correct exposure. Individual flowers were set lengthwise on a round frosted glass to view stem color differences. The top side of flowers were prepared to keep them upright by setting individual flowers stem-down into the barrel of a 10 mL syringe with the inside bore cut out. Flower preparations were set upon an 8×10” piece of glossy photo paper (Epson Premium Glossy Photo Paper) as a clean, white background, to view and compare the flower and stem color.
The callus images in petri plates were observed and photographed in a Leica MZ16-F (Leica Microsystems, Inc., Buffalo Grove, IL) stereo zoom light microscope equipped with a QImaging Retiga 2000 R fast cooled, digital color camera. For seed pods, the outer bracts were removed from one side before photographing.
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3

Digital Photography of Plant Phenotypes

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The photographs of the plants and fruits were recorded using a Nikon D7000 digital camera with an AF Micro Nikkor 60 mm 1:2.8 D lens or AF-S Nikkor 18–70 mm DX lens (Nikon Inc.) under tungsten lamps (Philips, 120 V, 300 W). The camera was set manually for all parameters including ISO sensitivity, focus, f-stop and time. A photography gray card was used as a reference to get the correct exposure. The agroinjection images and GUSPlus stained tomato fruits were observed and photographed in a Leica MZ16-F (Leica Microsystems, Inc.) stereo zoom light stereoscope equipped with a QImaging Retiga 2000 R fast cooled, digital color camera.
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