Acetylated sod2

Ara-C and DNR were purchased from the Sigma-Aldrich (St. Louis, MI, USA). NAC, Momordin-Ic and 3-TYP were consumed from Selleckchem (Houston, TX, USA). All compounds, except for in vivo studies, were reconstituted in the DMSO, stored at 100 mM stock concentrations in −80 °C, and used at the indicated doses as suggested by the vendor. Flow cytometry antibodies, Alexa Fluor 647 Rabbit anti-Active caspase 3 and APC-H7 Mouse anti-Human CD45 were purchased from BD pharmingen (San Jose, CA, USA). PE/Cy5 anti-Mouse CD45 (clone 30-F11) was consumed from BioLegend (San Diego, CA, USA). Immunoblotting antibodies, SUMO1, SIRT3, SENP1, Notch Activated Targets Antibody Sampler Kit including Notch1 (FL), MAML1, BPUSH and HES1, p-PI3K p85, t-PI3K p85, p-p38, t-p38, p-AKT and t-AKT were purchased from Cell Signaling Technology (Danvers, MA, USA). Acetylated SOD2 and SOD2 antibodies were purchased from Abcam (London, UK). Tubulin and β-actin antibodies were purchased from Proteintech (Rosemont, IL, USA).

Samples were collected from the area at risk after reperfusion for 3 hours. The expression of myocardial LC3B (1:1000, Cell Signaling Technology), SIRT3 (1:1000, CST, USA), acetylated SOD2 (lysine 68, 1:1000, Abcam, USA), SOD2 (1:1000, Santa Cruz, USA), COX4 (1:1000, CST, USA), p62 (1:1000, CST, USA), Beclin 1 (1:1000, CST, USA) and GAPDH (1:1000, CST, USA) was determined by immunoblotting.²⁸ The quantitative protein band density was measured by ImageJ 1.37.