Anti cd11b pe

Human serum albumin (HSA), methylglyoxal (MG), D-glucose, Histopaque, sodium citrate, 3,3′,5,5′-tetramethylbenzidine (TMB), phorbol 12-myristate 13-acetate (PMA), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), celestine blue B (CB), 4-chloro-1-naphtol (4-CN), Coomassie G-250, o-dianisidine, hydrogen peroxide (H₂O₂), horseradish peroxidase (HRP), luminol (Lum), lucigenin (Luc), Triton X-100 and all salts and solvents for the preparation of solutions were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acrylamide, methylene bis-acrylamide, tetramethylethylenediamine, ammonium persulphate, Tris and glycine were purchased from Panreac-AppliChem (Darmstad, Germany). Krebs-Ringer buffer solution was purchased from Merck (Kenilworth, NJ, USA). Anti-CD11b-PE, anti-CD63-APC, anti-CD45-FITC antibodies, non-fat dry milk (blotting grade blocker) and HRP-labeled anti-mouse IgG, nitrocellulose membrane were purchased from Bio-Rad (Hercules, CA, USA). Dextran T70 was purchased from Roth (Karlsruhe, Germany). May Grünwald’s Eosin–Methylene Blue solution and Romanowski Azur Eozin stain were purchased from ECOlab (Moscow, Russia).

Expression of cell surface markers was measured after blocking of unspecific binding sites with CD16/CD32Fc-Block (BD Biosciences, San Jose, CA, USA) by staining cells with anti-Gr-1-APC (BD Biosciences, San Jose, CA, USA, Clone RB6-8C5), anti-Ly6G (Gr-1)-PE (eBioscience, Clone RB6-8C5) anti-CD11b-PE (AbD serotec, Bio-Rad, München, Germany, Clone M1/70), anti-c-kit-APC (eBioscience, San Diego, CA, USA, Clone ACK2), anti-CXCR2-APC (Biolegend, San Diego, CA, USA, Clone TG11/CXCR2) or anti-CXCR4-APC (BD Biosciences, Heidelberg, Germany, Clone 2B11/CXCR4) followed by flow cytometry analysis on a FACS Calibur or Fortessa (BD Biosciences).