Acetylcholine

Analytical-grade chemicals were used for the experiments. Acetylcholine (ACh) [15 (link)], Norepinephrine (NE), and Potassium chloride (KCL) were purchased from BDH, Poole, England. Atorvastatin raw material was purchased from Polyfine Pharmaceutical Private Ltd., Peshawar, Pakistan. Fluvastatin of Novartis Pharma was purchased from the local market of Peshawar. Amlodipine raw material was obtained from Feroz-sons laboratories Pvt Ltd., Nowshera, Pakistan.
Less soluble raw materials in Krebs solution were suspended in 0.01% carboxy methyl cellulose (CM) using distilled water. To rule out any possible effects of CMC, a negative control in distilled water was conducted. Freshly prepared solutions and suspensions were used on the same day of the experiments.

Analytical grade chemicals were used throughout these experiments. Acetylcholine was purchased from BDH, Poole, England, which was used for the maintenance of tissues at quiescent doses. Rest of the chemicals were of E Merck grade. Stock solutions of all the chemicals were made in distilled water and the dilutions were made fresh in normal saline on the days of experiments.

Analytical grade chemicals were used in the experiments. Acetylcholine was purchased from BDH, Poole, England, which was used for maintenance of isolated tissues. Raw materials of rosuvastatin and atorvastatin as calcium salts were obtained from Ferozsons Laboratories Pvt. Ltd. Nowshera, Pakistan. Raw material of simvastatin was taken from Polyfine Pharmaceutical Industry, Peshawar. Fluvastatin of Novartis Pharma were purchased from local market of Peshawar, Pakistan. Raw materials, which had poor solubility in Tyrode’s solutions, were suspended in 0.01 % Carboxy Methyl Cellulose (CMC). However, a negative control of 0.01 % of CMC in deionized water was run to rule out any possible effects of CMC. All solutions and suspensions were freshly prepared on the same days of experiments.