Anti lag 3

Manufactured by R&D Systems

Anti-LAG-3 is a lab equipment product that functions as an antibody specific to the LAG-3 protein. LAG-3 is an immune checkpoint receptor that plays a role in regulating T cell activation and function. This product can be used for research purposes to study the LAG-3 signaling pathway and its implications in various biological processes.

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Lab products found in correlation

Anti phosphoser319 foxo1, by Bioss Antibodies (2 mentions) Live dead cell viability assay, by Thermo Fisher Scientific (2 mentions) Facsaria 2, by BD (2 mentions) Lsrfortessa, by BD (2 mentions) Anti pd 1 clone eh12.2h7, by BioLegend (1 mentions) Live dead fixable dead cell staining kit, by Thermo Fisher Scientific (1 mentions) Anti foxp3 clone pch101, by Thermo Fisher Scientific (1 mentions) Anti ctla 4 clone bni3, by BD (1 mentions) Anti il 10 jes3 9d7, by BioLegend (1 mentions)

3 protocols using anti lag 3

Comprehensive Immunophenotyping by Flow Cytometry

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For cellular isolation and immunophenotyping analyses, flow cytometry was performed using a BD LSRFortessa for analysis and a BD FACS Aria II for cell sorting. All analysis was carried out using FlowJo software (Treestar Inc). Cell viability was assessed using Live/Dead Cell Viability Assays (Life technologies). The following anti-human surface and intracellular antibodies and dyes were utilized: anti-CD4 (RPA-T4), anti-CD8 (RPA-T8), anti-CD25 (M-A251), anti-CD127 (HIL-7R-M21), anti-PD-1 (EH12.1), anti-PDL1 (MIH1) anti-IFNγ(B27), anti-KI67 (B56), anti-CCR6 (11A9), anti-CXCR3 (IC6), anti-CCR7 (3D12), anti-CXCR5 (RF8B2), V500 Annexin-V stain (all BD Biosciences); anti-TIM-3 (F38-2E2), anti-CTLA-4 (L3D10), anti-CD226 (TX25), anti-IL-10 (JES3-9D7) (all from Biolegend), anti-Foxp3 (PCH101), anti-IL-2 (MQ1-17H12), anti-ICOS (ISA3) (all from eBioscience), anti-LAG-3 (polyclonal goat IgG, R&D systems), anti-phosphoSer319 FoxO1 (polyclonal rabbit IgG, Bioss).

Goods B.A., Hernandez A.L., Lowther D.E., Lucca L.E., Lerner B.A., Gunel M., Raddassi K., Coric V., Hafler D.A, & Love J.C. (2017). Functional differences between PD-1+ and PD-1- CD4+ effector T cells in healthy donors and patients with glioblastoma multiforme. PLoS ONE, 12(9), e0181538.

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Comprehensive Immune Cell Profiling

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The following monoclonal antibodies and reagents were used: for surface staining, anti-CD25 (clone M-A251), anti-CD45RA (clone 5H9), anti-CXCR3 (clone 1C6/XCXR3), anti-CCR6 (clone 11A9) and anti-CTLA-4 (clone BNI3) from BD Biosciences, anti-CD127 (clone 17-1278) from eBioscience, anti-TIM-3 (clones F38-2E2 and 344823 from Biolegend and R&D systems respectively), anti-PD-1 (clone EH12.2H7) from Biolegend, anti-LAG-3 (polyclonal) from R&D systems and Live/Dead fixable dead cell staining kit from Invitrogen; for intracellular staining, anti-FoxP3 (clone PCH101) from eBioscience and anti-pSTAT3 (pY705) from BD Biosciences.

Gautron A.S., Dominguez-Villar M., de Marcken M, & Hafler D.A. (2014). Enhanced Suppressor Function of TIM-3+ FoxP3+ Regulatory T cells. European journal of immunology, 44(9), 2703-2711.

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Comprehensive Immunophenotyping by Flow Cytometry

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For immunophenotyping analyses, flow cytometry was performed with the following anti-human surface and intracellular antibodies and dyes: anti-CD4 (RPA-T4), anti-CD8 (RPA-T8), anti-CD25 (M-A251), anti-CD127 (HIL-7R-M21), anti-PD-1 (EH12.1), anti-PDL1 (MIH1) anti-IFN-γ (B27), anti-KI67 (B56), anti-CCR6 (11A9), anti-CXCR3 (IC6), anti-CCR7 (3D12), anti-CXCR5 (RF8B2), and V500 Annexin-V stain (all from BD Biosciences); anti-TIM-3 (F38-2E2), anti-CTLA-4 (L3D10), anti-CD226 (TX25), and anti-IL-10 (JES3-9D7) (all from Biolegend); anti-Foxp3 (PCH101), anti-IL-2 (MQ1-17H12), and anti-ICOS (ISA3) (all from eBioscience); anti-LAG-3 (polyclonal goat IgG, R&D Systems); and anti-phosphoSer319 FoxO-1 (polyclonal rabbit IgG, Bioss). Cell viability was assessed using Live/Dead Cell Viability Assays (Life technologies). Samples were run using a BD LSRFortessa for all analysis, and FACS sorting was carried out on a BD FACS Aria II. FlowJo software (TreeStar Inc.) was used for data collection and analysis.

Lowther D.E., Goods B.A., Lucca L.E., Lerner B.A., Raddassi K., van Dijk D., Hernandez A.L., Duan X., Gunel M., Coric V., Krishnaswamy S., Love J.C, & Hafler D.A. (2016). PD-1 marks dysfunctional regulatory T cells in malignant gliomas. JCI Insight, 1(5), e85935.

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