Venous blood samples were obtained in the early morning (9–10 am) on the 3rd to 5th days of the menstrual cycle, which was spontaneous or induced by progesterone. Fasting plasma glucose levels were measured by photometric analysis (Abbott Architect C16000 AutoAnalyzer, Abbott Diagnostics, USA). Fasting serum insulin, follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin, dehydroepiandrosterone sulfate (DHEAS), total testosterone, and thyroid stimulating hormone (TSH) levels were measured by chemiluminescent microparticle enzyme immunoassay (Abbott Architect i2000, Abbott Diagnostics, USA). Serum 17 hydroxyprogesterone (17-OHP) and free testosterone levels were specified by radioimmunoassay and high-sensitive C-reactive protein (hs-CRP) concentrations were measured by immunoturbidimetry (Abbott Architect C16000 AutoAnalyzer, Abbott Diagnostics, USA). The Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) index was calculated as follows:
Architect c16000 autoanalyzer
Manufactured by Abbott
Sourced in United States
The Architect c16000 Autoanalyzer is a fully automated clinical chemistry analyzer designed for high-volume testing in a laboratory setting. It is capable of performing a wide range of clinical chemistry assays, including tests for proteins, enzymes, lipids, and other analytes.
Automatically generated - may contain errors
Lab products found in correlation
Cell dyn 3700, by Abbott (5 mentions)
Vivid s6, by GE Healthcare (2 mentions)
Cell dyn ruby, by Abbott (2 mentions)
Image 800, by Beckman Coulter (2 mentions)
Architect i2000, by Abbott (1 mentions)
Advia centaur xp immunoassay system, by Siemens (1 mentions)
Dca 2000 system, by Siemens (1 mentions)
Sandwich enzyme linked immunosorbent assay kit, by Cloud-Clone (1 mentions)
Human adiponectin elisa, by BioVendor (1 mentions)
Cobas 6000, by Roche (1 mentions)
Au5400 automatic biochemical analyzer, by Olympus (1 mentions)
Sta compact max, by Diagnostica Stago (1 mentions)
Cobas e601 analyzer, by Roche (1 mentions)
Xn 1000, by Sysmex (1 mentions)
Vacuette, by Greiner (1 mentions)
Architect c16000, by Abbott (1 mentions)
21 protocols using architect c16000 autoanalyzer
1
Hormonal and Metabolic Profile Assessment
2
Serum Biomarker Levels Determination
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The serum LDH, ferritin, AST, ALT, and CK-MB levels were detected with an Abbott architect c16000 autoanalyzer (Abbott Diagnostics Inc, Park City, IL, USA). The CRP levels were determined by the turbidimetric method in the same autoanalyzer.
3
Measurement of Antioxidant and Oxidant Status in Rat Kidneys
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The kidneys were removed from the abdominal cavities of the rats. Then, they were washed in ice-cold phosphate-buffered saline (PBS). All kidney samples were weighed and homogenized in PBS (pH 7.4) for five minutes. The ratio of tissue weight to homogenization buffer was 1:10. The homogenates were centrifuged at 2717 × g for 20 min at 4 °C. Part of the resulting supernatants was used for the determination of Total Antioxidant Status (TAS) and Total Oksidant Status (TOS) by autoanalyzer Architect c16000 Autoanalyzer, Abbott Diagnostics, Waltham, MA [21 (link),22 (link)].
4
Biochemical Assays for Metabolic Markers
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Serum fasting blood glucose, triglyceride (TG), total cholesterol (C), HDL-C, and LDL-C concentrations were determined with kits by Architect c16000 autoanalyzer (Abbott Diagnostics, USA). Vitamin B12 and folic acid concentrations were determined with Advia Centaur XP immunoassay system (Siemens, Germany) with kits by Advia Centaur XP immunoassay system.
5
Serum Biomarkers in Patient Drug Distributions
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The distribution of used drugs in patients.
In the patients treated previously, the measurements before the diagnosis were taken into consideration. Venous blood samples were collected after an overnight fast. Serum was immediately prepared and stored in aliquots at -80°C before analysis. The levels of fasting glucose, blood urea nitrogen, creatinine, uric acid, total protein, albumin, triglyceride, total cholesterol, and high-density lipoprotein cholesterol were determined using commercially available assay kits (Abbott Diagnostics, Abbott Park, Illinois, USA) with a Architect c16000 auto-analyzer (Abbott Diagnostics, Abbott Park, Illinois, USA). 24-h urinary protein excretion (UPE) was measured by immunoassay (DCA 2000 system, Siemens AG, Munich, Germany). High-sensitivity C-reactive protein (hs-CRP) was measured on an Abbott Architect i1000 analyzer. We calculated estimated glomerular filtration rate (eGFR) using 2009 the CKD Epidemiology Collaboration (CKD-EPI) creatinine equation. [ 20 ] Serum SPON2 levels were measured using a commercial sandwich enzyme-linked immunosorbent assay kit from Cloud-Clone Corp (Houston, TX, USA).
In the patients treated previously, the measurements before the diagnosis were taken into consideration. Venous blood samples were collected after an overnight fast. Serum was immediately prepared and stored in aliquots at -80°C before analysis. The levels of fasting glucose, blood urea nitrogen, creatinine, uric acid, total protein, albumin, triglyceride, total cholesterol, and high-density lipoprotein cholesterol were determined using commercially available assay kits (Abbott Diagnostics, Abbott Park, Illinois, USA) with a Architect c16000 auto-analyzer (Abbott Diagnostics, Abbott Park, Illinois, USA). 24-h urinary protein excretion (UPE) was measured by immunoassay (DCA 2000 system, Siemens AG, Munich, Germany). High-sensitivity C-reactive protein (hs-CRP) was measured on an Abbott Architect i1000 analyzer. We calculated estimated glomerular filtration rate (eGFR) using 2009 the CKD Epidemiology Collaboration (CKD-EPI) creatinine equation. [ 20 ] Serum SPON2 levels were measured using a commercial sandwich enzyme-linked immunosorbent assay kit from Cloud-Clone Corp (Houston, TX, USA).
6
Comprehensive Metabolic Panel Assay
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BUN, creatinine, total cholesterol, triglycerides, and low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterol were measured with commercially available assay kits (Abbott Diagnostics, Abbott Park, IL) and an Architect C16000 auto-analyzer (Abbott Diagnostics).
7
Serum Adipokine and Metabolic Biomarkers
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The concentrations of the adipokines, adiponectin and leptin in serum samples were assayed at the “Nutrition Research Unit” Laboratory at King Fahd Medical Research Center. Serum adiponectin was measured using “Biovendor human adiponectin ELISA” high sensitivity kit, while serum leptin was assayed using DRG®Leptin ELISA kit. Both assays were solid phase enzyme-linked immunosorbent assay, and were carried out according to the manufacturers’ procedure. Absorbance was measured at 450±10 nm using a microplate reader (Biokit, ELX800- USA).
Serum biochemical and remaining endocrine parameters were assayed in accredited Clinical Chemistry laboratory at the National Guard Hospital, King Abdulaziz Medical City-Jeddah. For serum glucose, liver enzymes, albumin and total protein tests Abbott architect c16000 auto-analyzer (spectrophotometric method) was used. On the same auto-analyzer, the method of immunoturbidimetric determination technique was utilized to measure hsCRP. Insulin measurement was done on Abbott Architect i2000 auto-analyser by using the method of a chemiluminescent microparticle immunoassay (CMIA). Growth hormone was measured by using chemiluminescent immunometric method on immulite 1000 analyzer.
Serum biochemical and remaining endocrine parameters were assayed in accredited Clinical Chemistry laboratory at the National Guard Hospital, King Abdulaziz Medical City-Jeddah. For serum glucose, liver enzymes, albumin and total protein tests Abbott architect c16000 auto-analyzer (spectrophotometric method) was used. On the same auto-analyzer, the method of immunoturbidimetric determination technique was utilized to measure hsCRP. Insulin measurement was done on Abbott Architect i2000 auto-analyser by using the method of a chemiluminescent microparticle immunoassay (CMIA). Growth hormone was measured by using chemiluminescent immunometric method on immulite 1000 analyzer.
8
Hematological and Metabolic Markers Protocol
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On admission, we obtained venous blood from all patients. The NLR was calculated as the ratio of the neutrophils and lymphocytes, both obtained from the same automated blood sample at admission. Baseline PLR was measured by dividing the platelet count by the lymphocyte count. Hematologic indices were measured by an automated hematology analyzer system (Abbott Cell-Dyn 3700; Abbott Laboratory, Abbott Park, Illinois). Absolute cell counts were utilized to perform subsequent analyses. Total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides, glucose, uric acid level (UA) and creatinine levels were measured with the Abbott Architect C16000 autoanalyzer (Abbott Laboratory). Fasting lipid panels were obtained after an overnight fast.
9
Predictive Biomarkers for Acute Myocardial Infarction
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Venous blood samples were collected when the patient initially presented to the emergency department or intensive coronary care unit (ICCU) before primary PCI. Hematologic indices were measured by an automated hematology analyzer system (Abbott Cell-Dyn 3700; Abbott Laboratory, Abbott Park, Illinois). Absolute cell counts were utilized to perform subsequent analyses. Baseline PLR was measured by dividing the platelet count by the lymphocyte count. Total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides, glucose, UA, and creatinine levels were measured with the Abbott Architect C16000 autoanalyzer (Abbott Laboratory). Creatinine kinase-MB (CK-MB) levels were measured with a Beckman Access analyzer (USA). Troponin I levels were measured with a Beckman Image 800 analyzer (USA). Normal UA levels were defined as ranging between 2.6-7.2 mg/dL. Transthoracic two-dimensional echocardiography was performed upon admission to the ICCU to determine left ventricular ejection fraction (LVEF), left ventricular systolic diameter (LVSD), left ventricular diastolic diameter (LVDD), and left atrial diameter (Vivid S6, GE Medical Systems, Horten, Norway).
10
Metabolic Biomarker Profiling Protocol
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After fasting overnight, a venous blood sample (10 mL) was obtained. Total cholesterol, high density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL), triglycerides, glucose, and creatinine levels were measured with the Abbott Architect C16000 auto-analyzer (Abbott Park, Abbott Diagnostics, USA). Serum insulin was determined by a commercially available assay kit (Roche Cobas 6000, Roche-Hitachi Diagnostics, Germany). Insulin-resistance was estimated using the homeostasis model assessment as an index of insulin resistance (HOMA-IR), defined as follows: (fasting glucose mg/dL x fasting insulin µU/mL) / 405 (17 (link)). The cut-off value was taken as 2.7 for HOMA-IR (18 ). Hematologic parameters were measured by an automated hematology analyzer system (Abbott Cell-Dyn Ruby; Abbott Diagnostics, USA). Absolute cell counts were utilized to perform subsequent analyses. The serum concentration of C-reactive protein was evaluated using the high-sensitivity immunochemistry system, the nephelometric method (Image 800, Beckman Coulter, USA).
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