Log In Sign up
The largest database of trusted experimental protocols

Anti phospho threonine

Manufactured by Merck Group
Visit Supplier

Anti-phospho-threonine is a laboratory reagent used to detect and quantify phosphorylated threonine residues in proteins. It is a specific antibody that binds to threonine residues that have been modified by the addition of a phosphate group, enabling researchers to study protein phosphorylation, a key regulatory mechanism in biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Ribonucleosides, by Merck Group (1 mentions) Nocodazole, by Merck Group (1 mentions) Tetracycline, by Merck Group (1 mentions) U 13c glucose, by Cambridge Isotopes (1 mentions) Anti gst, by Proteintech (1 mentions) Anti actin, by Proteintech (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Glucose 6 phosphate (g6p), by Merck Group (1 mentions) Anti cdk2, by Proteintech (1 mentions) Anti g6pd, by Proteintech (1 mentions) Anti phospho h3ser10, by Merck Group (1 mentions) Anti cyclin b1, by Proteintech (1 mentions) Anti plk1, by Merck Group (1 mentions) 2 13c glucose, by Cambridge Isotopes (1 mentions) Anti g6pd, by Abcam (1 mentions) Mouse igg, by Santa Cruz Biotechnology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti ha, by Merck Group (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions)

2 protocols using anti phospho threonine

1

Plk1 Regulation of Pentose Phosphate Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies and reagents used in the study are listed as following: anti-Plk1 (Sigma-Aldrich, P5998, 1:10,000), anti-Flag (Sigma, F3165, 1:5000), anti-HA (Sigma, H9658, 1:5000), anti-G6PD (Protein-tech, 25413-1-AP, 1:1000), anti-G6PD (Abcam, ab993, 1:1000), anti-glutathione S-transferase (anti-GST; Protein-tech, 10000-0-AP, 1:5000), anti-phospho-H3Ser10 (Millipore, 06-570, 1:1000), anti-cyclin B1 (Protein-tech, 55004-1-AP, 1:1000), anti-phospho-threonine (Millipore, AB1607, 1:1000), anti-PGLS (Santa Cruz, SC-398833, 1:1000), anti-6PGD (Protein-tech, 14718-1-AP, 1:1000), anti-CDK2 (Protein-tech, 10122-1-AP, 1:1000), rabbit IgG (Protein-tech), mouse IgG (Santa Cruz), and anti-Actin (Protein-tech, 60008-1-Ig, 1:5000). NADP+, NADPH, GSH, G6P, 6PG, NAC, Dox, tetracycline, HU, propidium iodide, nocodazole, 4,6-diamidino-2-phenylindole (DAPI), casein, D2O, ribonucleosides, and deoxyribonucleosides were purchased from Sigma-Aldrich; R5P, nucleotides (AXP, GXP, CXP, and UXP, X = M, D, and T) were purchased from Sangon Biotech. BI2536 was purchased from Selleck Corporation, PP2A was from Millipore, and human recombinant Plk1 was from Sino biological company. [2-13C]-glucose and [U-13C]-glucose were from Cambridge Isotope Laboratories. γ-32P ATP was from China Isotope & Radiation Corporation.
Ma X., Wang L., Huang D., Li Y., Yang D., Li T., Li F., Sun L., Wei H., He K., Yu F., Zhao D., Hu L., Xing S., Liu Z., Li K., Guo J., Yang Z., Pan X., Li A., Shi Y., Wang J., Gao P, & Zhang H. (2017). Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. Nature Communications, 8, 1506.
+ Open protocol
+ Expand
2

Western Blot Analyses using Multiple Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analyses were performed as we described previously11 (link)17 (link)18 (link)20 (link)23 (link), using the following antibodies: anti-fortilin
(polyclonal antibody, MBL International, Woburn, MA, used for Fig. 1A,B, Fig. 2A, Fig.
3C, Fig. S2B, Fig. S2C, Fig.
S2D and Fig. S4B; monoclonal antibody [Clone 2C4], Abnova, Taiwan used
for Fig. 1F, Fig. 3A and Fig. S4F), anti-hemagglutinin (HA;
16B12, Bethyl Laboratory, Montgomery, TX), anti-FLAG (M2, Sigma), anti-human p53
(DO1, Santa Cruz), anti-PRX1 (goat polyclonal, PAB11441, Abnova, Taiwan),
anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 6C5, Fitzgerald),
anti-ubiquitin (BostonBiochem, Cambridge, MA), and anti-phosphothreonine
(Millipore, Billerica, MA) antibodies.
Chattopadhyay A., Pinkaew D., Doan H.Q., Jacob R.B., Verma S.K., Friedman H., Peterson A.C., Kuyumcu-Martinez M.N., McDougal O.M, & Fujise K. (2016). Fortilin potentiates the peroxidase activity of Peroxiredoxin-1 and protects against alcohol-induced liver damage in mice. Scientific Reports, 6, 18701.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!