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Eif2α l57a5

Manufactured by Cell Signaling Technology
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EIF2α (L57A5) is an antibody product from Cell Signaling Technology. It is used to detect the expression of the eukaryotic translation initiation factor 2 subunit alpha (eIF2α) protein.

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Lab products found in correlation

Phospho eif2α, by Cell Signaling Technology (1 mentions) Stat3 79d7, by Cell Signaling Technology (1 mentions) β actin 13e5, by Cell Signaling Technology (1 mentions) Phospho sapk jnk 81e11, by Cell Signaling Technology (1 mentions) Phospho c jun d47g9, by Cell Signaling Technology (1 mentions) Gadd153 f 168, by Santa Cruz Biotechnology (1 mentions) Jnk2 n 18, by Santa Cruz Biotechnology (1 mentions) C jun 60a8, by Cell Signaling Technology (1 mentions) Phospho stat3 d3a7, by Cell Signaling Technology (1 mentions) Pkr b 10, by Santa Cruz Biotechnology (1 mentions) Phospho t446 pkr, by Abcam (1 mentions) Penta his, by Qiagen (1 mentions) Phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Pkr d7f7, by Cell Signaling Technology (1 mentions) Western star chemiluminescent detection system, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

2 protocols using eif2α l57a5

1

Hepatocyte Stress Signaling Analysis

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Total liver lysates were analyzed by immunoblotting using antibodies against HBsAg (20-HR20, Fitzgerald), GADD153 (F-168, Santa Cruz), phospho-PERK (16F8, Cell Signaling), PERK (H-300, Santa Cruz), phospho-eIF2α (119A11, Cell Signaling), eIF2α (L57A5, Cell Signaling), β-actin (13E5, Cell Signaling), JNK2 (N-18, Santa Cruz), c-Jun (60A8, Cell Signaling), phospho-c-Jun (D47G9, Cell Signaling), phospho-SAPK/JNK (81E11, Cell Signaling), STAT3 (79D7, Cell Signaling), phospho-STAT3 (D3A7, Cell Signaling).
Churin Y., Roderfeld M., Stiefel J., Würger T., Schröder D., Matono T., Mollenkopf H.J., Montalbano R., Pompaiah M., Reifenberg K., Zahner D., Ocker M., Gerlich W., Glebe D, & Roeb E. (2014). Pathological Impact of Hepatitis B Virus Surface Proteins on the Liver Is Associated with the Host Genetic Background. PLoS ONE, 9(3), e90608.
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2

Western Blot Immunodetection Protocol

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With the exception of pulldown assays (above), lysates for immunoblot assays were prepared by washing the monolayer with phosphate-buffered saline and lysing the cells with 2% SDS. After sonication to disrupt the cellular DNA, proteins were separated by 10% SDS-PAGE, transferred to a PVDF membrane (Millipore), and probed with the indicated antibodies using the Western Star chemiluminescent detection system (Applied Biosystems). Antibodies used in these experiments include eIF2α L57A5 (#2103), phospho-eIF2α Ser51 (#3597), and PKR D7F7 (#12297), all from Cell Signaling Technology, PKR B-10 (sc6282, Santa Cruz Biotechnology), Penta-His (Qiagen), phospho-PKR T446 (ab32026, AbCam), and Actin (A2066, Sigma). Rabbit antiserum that recognizes the TRS1/IRS1 dsRNA binding domain (anti-p999) has been described previously (Marshall et al., 2009 (link)).
Braggin J.E., Child S.J, & Geballe A.P. (2015). Essential Role of Protein Kinase R Antagonism by TRS1 in Human Cytomegalovirus Replication. Virology, 489, 75-85.
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