Gentra purgene blood kit

Manufactured by Qiagen

Sourced in United States

The Gentra Purgene Blood Kit is a product designed for the purification of DNA from whole blood samples. It provides a simple and efficient method for extracting high-quality genomic DNA from blood specimens.

Automatically generated - may contain errors

Lab products found in correlation

Dnaesy blood and tissue kit, by Qiagen (2 mentions) Quick rna ffpe kit, by Zymo Research (2 mentions) Mirneasy mini kit, by Qiagen (2 mentions) Dneasy blood and tissue kit, by Qiagen (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions) Sherlock ax dna isolation kit, by A&A Biotechnology (1 mentions) Dreamtaq dna polymerase, by Thermo Fisher Scientific (1 mentions) Nebnext ultra 2 fs dna library prep kit for, by Illumina (1 mentions) Novaseq 6000, by Illumina (1 mentions) 60k cytosure constitutional v3 microarray, by Oxford Gene Technology (1 mentions) Taqman allelic discrimination assay, by Thermo Fisher Scientific (1 mentions) Imr90 fibroblasts, by American Type Culture Collection (1 mentions) Taqman snp genotyping assay, by Thermo Fisher Scientific (1 mentions) 7900ht fast real time pcr system, by Thermo Fisher Scientific (1 mentions) Humanomni2.5 beadchip, by Illumina (1 mentions) Ez dna methylation kit, by Zymo Research (1 mentions) Humanmethylation450 beadchip, by Illumina (1 mentions) Qiaamp dna investigator kit, by Qiagen (1 mentions) Quick dna urine kit, by Zymo Research (1 mentions) Prepit l2p, by DNA Genotek (1 mentions)

9 protocols using gentra purgene blood kit

Prenatal Genetic Screening and Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For invasive prenatal studies, DNA was extracted from amniocytes using the Sherlock AX DNA isolation kit (A&A Biotechnology, Gdansk, Poland), according to manufacturer’s instructions. Array comparative genomic hybridization (aCGH) in the fetus was performed using the 60K CytoSure Constitutional v3 microarray (Oxford Gene Technology, Oxford, UK).
For further genetic testing, DNA was extracted from the peripheral blood of the newborn and his parents using Gentra Purgene Blood Kit (Qiagen, Germantown, MD, USA). Parental and proband DNA samples were tested for the presence of the CNV deletion using junction-specific PCR with DreamTaq DNA Polymerase (Thermo Scientific, Waltham, MA, USA), followed by Sanger sequencing to map the deletion breakpoints. To determine the parental origin of the detected CNV deletion, trio-based genome sequencing (GS) was performed using NEBNext^® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs, Inc. Ipswich, MA, USA) and paired-end sequenced (2 × 150 bp) on NovaSeq 6000 (Illumina, San Diego, CA, USA). The parental origin of the observed chromosomal abnormality was determined by analyzing the informative single-nucleotide polymorphisms (SNPs) within the deletion region.

Bzdęga K., Kutkowska-Kaźmierczak A., Deutsch G.H., Plaskota I., Smyk M., Niemiec M., Barczyk A., Obersztyn E., Modzelewski J., Lipska I., Stankiewicz P., Gajecka M., Rydzanicz M., Płoski R., Szczapa T, & Karolak J.A. (2023). Prenatal Detection of a FOXF1 Deletion in a Fetus with ACDMPV and Hydronephrosis. Genes, 14(3), 563.

+ Open protocol

+ Expand

Pharmacogenetic Genotyping of Transporter and Metabolizing Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted from whole blood using the Gentra Purgene Blood Kit (Qiagen, Toronto, Ontario, Canada). The following TaqMan allelic discrimination assays (Applied Biosystems, Carlsbad, CA, USA) were used for genotyping: ABCB1 (c.3435C>T, rs1045642), ABCG2 (c.421C>A, rs2231142; c.34G>A, rs2231137), ABCC2 (c.-24C>T, rs717620; c.1249G>A, rs2273697), ABCC5 (T>C, rs562), SLCO1B1*1b (c.388A>G, rs2306283), SLCO1B1*5 (c.521T>C, rs4149056), SLCO1B3 (g.699G>A, rs7311358), UGT1A1*28 (TA(6/7), rs8175347), CES1 (g.14506G>A, rs71647871; g.27467A>C, rs2244613), CYP3A4*22 (intron 6 C>T, rs35599367), and CYP3A5*3 (g.6986A>G, rs776746). Hardy–Weinburg equilibrium was assessed for all genotypes using the χ² goodness-of-fit test.

Teft W.A., Welch S., Lenehan J., Parfitt J., Choi Y.H., Winquist E, & Kim R.B. (2015). OATP1B1 and tumour OATP1B3 modulate exposure, toxicity, and survival after irinotecan-based chemotherapy. British Journal of Cancer, 112(5), 857-865.

+ Open protocol

+ Expand

Protocol for Extracting Nucleic Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood, saliva, and frozen or formalin-fixed paraffin-embedded (FFPE) lung biopsy or autopsy samples were received after obtaining written informed consent from the patients’ parents. DNA was extracted from blood and saliva using Gentra Purgene Blood Kit (Qiagen, Germantown, MD, USA), and from frozen lung tissue using DNaesy Blood and Tissue Kit (Qiagen). RNA from frozen lung samples, and cultured IMR-90 fibroblasts (ATCC, Manassas, VA, USA) was extracted using miRNeasy Mini Kit (Qiagen). RNA from FFPE lung tissues obtained by biopsy or acquired at autopsy was extracted using Quick-RNA FFPE Kit (Zymo Research, Irvine, CA, USA).

Szafranski P., Liu Q., Karolak J.A., Song X., de Leeuw N., Faas B., Gerychova R., Janku P., Jezova M., Valaskova I., Gibbs K.A., Surrey L.F., Poisson V., Bérubé D., Oligny L.L., Michaud J.L., Popek E, & Stankiewicz P. (2019). Association of rare non-coding SNVs in the lung-specific FOXF1 enhancer with a mitigation of the lethal ACDMPV phenotype. Human genetics, 138(11-12), 1301-1311.

+ Open protocol

+ Expand

FTO Genotyping from Blood Samples

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted from peripheral blood leukocytes from EDTA whole blood using the Gentra Purgene Blood Kit (QIAGEN Science, Germantown, MD, USA). The genotyping of rs9939609 FTO was performed using 7900HT Fast Real-Time PCR System and predesigned TaqMan SNP Genotyping Assays (Life Technologies/ Thermo Fisher Scientific, Waltham, MA, USA) specified for the SNP. A positive and a negative control were included on each sample tray [24 (link)].

de Soysa A.K., Langaas M., Jakic A., Shojaee-Moradie F., Umpleby A.M., Grill V, & Mostad I.L. (2021). The fat mass and obesity-associated (FTO) gene allele rs9939609 and glucose tolerance, hepatic and total insulin sensitivity, in adults with obesity. PLoS ONE, 16(3), e0248247.

+ Open protocol

+ Expand

Vitamin D Pathway Genetic Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted from peripheral blood using a commercial kit (Gentra Purgene Blood Kit (Qiagen, Gemantown, ML USA). Genotyping tests were developed using Illumina Human Omni 2.5 BeadChip (San Diego, CA, USA). Five genes in the vitamin D pathway were used in this study, VDR, CYP2R1, CYP27B1, CYP24A1 and CG/DBP. The VDR genetic information was extracted from 48,235,320 to 48,298,814 (Location: NC_000012.12) position at chromosome 12; CYP2R1 information was extracted from 1,489,951 to 14,913,874 (location: NC_000011.10) position at chromosome 11; CYP24A1 information was extracted from 52,769,985 to 52,790,516 (location: NC 000,020.11) position at chromosome 20; CYP27B1 information was extracted from 58,156,117 to 58,160,976 (location: NC 000012.12) position at chromosome 12 and DBP information was extracted from 49,133,817 to 49,140,639 (location: NC000019.10) position at chromosome 19. Quality control was carried out in PLINK version 1.07. SNVs were excluded if MAF (minor allele frequency) was less than 1%, imbalance of Hardy–Weinberg equilibrium with P value less than 10⁻⁴ and percentage of missing loci more than 1%.

Galvão A.A., de Araújo Sena F., Andrade Belitardo E.M., de Santana M.B., Costa G.N., Cruz Á.A., Barreto M.L., Costa R.D., Alcantara-Neves N.M, & Figueiredo C.A. (2020). Genetic polymorphisms in vitamin D pathway influence 25(OH)D levels and are associated with atopy and asthma. Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology, 16, 62.

+ Open protocol

+ Expand

DNA Extraction from Blood and FFPE Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted from peripheral blood or FFPE lung tissue using Gentra Purgene Blood Kit (Qiagen, Germantown, MD, USA) and DNeasy Blood and Tissue Kit (Qiagen), respectively.

Szafranski P., Kośmider E., Liu Q., Karolak J.A., Currie L., Parkash S., Kahler S.G., Roeder E., Littlejohn R.O., DeNapoli T.S., Shardonofsky F.R., Henderson C., Powers G., Poisson V., Bérubé D., Oligny L., Michaud J.L., Janssens S., De Coen K., Van Dorpe J., Dheedene A., Harting M.T., Weaver M.D., Khan A.M., Tatevian N., Wambach J., Gibbs K.A., Popek E., Gambin A, & Stankiewicz P. (2018). LINE-and Alu-containing genomic instability hotspotat 16q24.1 associated with recurrent and nonrecurrent CNV deletions causative for ACDMPV. Human mutation, 39(12), 1916-1925.

+ Open protocol

+ Expand

DNA Methylation Profiling with Illumina BeadChip

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was extracted manually from the buffy coat fractionated from EDTA whole blood using the Gentra Purgene blood kit (QIAGEN Science, MD, USA). DNA samples were quantified using both NanoDrop Spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and PicoGreen DNA methods. Samples (750 ng) were bisulfite converted using the EZ DNA Methylation Kit (Zymo Research, CA, USA). DNA samples were hybridized to the BeadChip arrays by the Genomics Core Facility (GCF) at the Norwegian University of Science and Technology (NTNU), Trondheim, Norway. HumanMethylation450 BeadChips (Illumina, San Diego, CA) were processed according to the manufacturer’s instructions. The BeadChip interrogates 485,000 methylation sites at single-nucleotide resolution. Annotations were done using the UCSC Genome Browser on Human Feb. 2009 (GRCh37/hg19) Assembly. BeadChip batch effects were present, and illustrated using PCA (see Supplementary Fig. 1), although not influenced by BMI since plate location of individuals with high or low BMI was unknown to the laboratory personnel.

Kvaløy K., Page C.M, & Holmen T.L. (2018). Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study. Scientific Reports, 8, 16330.

+ Open protocol

+ Expand

Comprehensive Nucleic Acid Extraction Methods

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood DNA was extracted using Gentra Purgene Blood Kit (Qiagen, Germantown, MD). DNA from urine was extracted 24 hours after collection using the Quick‐DNA Urine Kit (Zymo Research, Irvine, CA). The prepIT‐L2P (DNA Genotek, Ottawa, Canada) reagent was used to isolate DNA from buccal cells and saliva. The QIAamp DNA Investigator Kit (Qiagen) was used to extract DNA from hair follicles and nail clippings from fingers and toes. All procedures were followed to the manufacturer's protocols.

Yıldız Bölükbaşı E., Karolak J.A., Szafranski P., Gambin T., Willard N., Abman S.H., Galambos C., Kinsella J.P, & Stankiewicz P. (2022). High‐level gonosomal mosaicism for a pathogenic non‐coding CNV deletion of the lung‐specific FOXF1 enhancer in an unaffected mother of an infant with ACDMPV. Molecular Genetics & Genomic Medicine, 10(11), e2062.

+ Open protocol

+ Expand

DNA and RNA Extraction from Diverse Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA was isolated from blood and saliva using Gentra Purgene Blood Kit (Qiagen, Germantown, MD), or from frozen lung tissue using DNeasy Blood and Tissue Kit (Qiagen). RNA from frozen lung or FFPE lung tissues was extracted using miRNeasy Mini Kit (Qiagen) or Quick-RNA FFPE Kit (Zymo Research, Irvine, CA), respectively. DNA and RNA from cultured IMR-90 cells was isolated using DNaesy Blood and Tissue Kit (Qiagen) and miRNeasy Mini Kit (Qiagen), respectively.

Karolak J.A., Gambin T., Szafranski P., Maywald R.L., Popek E., Heaney J.D, & Stankiewicz P. (2021). Perturbation of semaphorin and VEGF signaling in ACDMPV lungs due to FOXF1 deficiency. Respiratory Research, 22, 212.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!