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3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reduction assay components

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3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components are a colorimetric assay used to measure cell metabolic activity. The assay involves the reduction of the tetrazolium dye MTT to insoluble purple formazan crystals by metabolically active cells.

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Lab products found in correlation

Rpmi 1640 medium and supplements for cell proliferation, by Merck Group (4 mentions) N n n n tetramethylene diamine temed, by Merck Group (2 mentions) Gallium nitrate hydrate, by Merck Group (1 mentions) Strontium nitrate, by Merck Group (1 mentions) Magnesium nitrate hexahydrate, by Merck Group (1 mentions) Ammonium dihydrogen phosphate nh4h2po4, by Merck Group (1 mentions) Urea nh2 2co, by Thermo Fisher Scientific (1 mentions) Zinc nitrate hexahydrate, by Merck Group (1 mentions) Initiator ammonium persulfate, by Merck Group (1 mentions) Sodium bicarbonate nahco3, by Merck Group (1 mentions) Curcumin from curcuma longa, by Merck Group (1 mentions) Potassium persulfate, by Merck Group (1 mentions) Resveratrol rsv, by Merck Group (1 mentions) Alginate, by Merck Group (1 mentions) N ethyl n 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Merck Group (1 mentions) N hydroxysuccinimide, by Merck Group (1 mentions)

4 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reduction assay components

1

Gelatin-Based Hydrogel for Cell Proliferation

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2-Hydroxyethyl methacrylate (H), gelatin (G, Type A), ethylene glycol dimethacrylate (EGDMA), 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC), ammonium persulfate (APS), and N,N,N’,N’-tetramethylene diamine (TEMED) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Manuka honey (MHo) was supplied by Haines Apiaries Ltd. (ArtisanHoney®, Kaitaia, New Zealand). RPMI-1640 medium and supplements for cell proliferation, as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components, were purchased from Sigma-Aldrich, St. Louis, MO, USA. All syntheses were performed in deionized water. All experiments were performed using lab-produced, ultra-distilled water.
Tomić S.L., Vuković J.S., Babić Radić M.M., Filipović V.V., Živanović D.P., Nikolić M.M, & Nikodinovic-Runic J. (2023). Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. Polymers, 15(3), 589.
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2

Biofunctional Biomaterials for Tissue Regeneration

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2-Hydroxyethyl methacrylate monomer (HEMA), gelatin from porcine skin (G, Type B), sodium alginate (A), ethyleneglycol dimethacrylate (EGDMA), as well as crosslinker 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC), potassium persulfate (PPS), N,N,N′,N′-tetramethylene diamine (TEMED), Pluronic F-172, sodium bicarbonate (NaHCO3) were purchased from Sigma-Aldrich, St. Louis, MO, USA. RPMI-1640 medium and supplements for cell proliferation as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components were purchased from Sigma-Aldrich. All syntheses were performed in deionized water. Materials used for the hydroxyapatite synthesis and doping were calcium nitrate pentahydrate (Ca(NO3)2×5H2O) (Sigma-Aldrich), magnesium nitrate hexahydrate (Mg(NO3)2×6H2O), strontium nitrate (Sr(NO3)2), gallium nitrate hydrate (Ga(NO3)3×H2O), zinc nitrate hexahydrate (Zn(NO3)2×6H2O), ammonium dihydrogen phosphate (NH4H2PO4) were obtained from Sigma-Aldrich, and urea ((NH2)2CO) was purchased from Alfa Aesar (Haverhill, MA, USA), and all were of analytical grade. All experiments were performed using lab-produced, ultra-distilled water.
Babić Radić M.M., Filipović V.V., Vuković J.S., Vukomanović M., Rubert M., Hofmann S., Müller R, & Tomić S.L. (2022). Bioactive Interpenetrating Hydrogel Networks Based on 2-Hydroxyethyl Methacrylate and Gelatin Intertwined with Alginate and Dopped with Apatite as Scaffolding Biomaterials. Polymers, 14(15), 3112.
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3

Graphene Oxide-Reinforced Bioactive Foam

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The monomer 2-hydroxyethyl methacrylate (HEMA, 99%) and polymers: gelatin from porcine skin (G, Type A) and sodium alginate (A) and nanomaterial graphene oxide (GO, nanocolloids, dispersion in water) were supplied from Sigma-Aldrich, Germany. Cross-linking agents: N,N′-methylenebis(acrylamide) (BIS) and glutaraldehyde, initiator-ammonium persulfate (APS), activator-N,N,N′,N′-tetramethylene diamine (TEMED), foaming stabilizer-Pluronic F-172 were obtained from Sigma-Aldrich, Germany. Foaming agents-sodium bicarbonate (NaHCO3) and curcumin (from Curcuma longa) were purchased from Sigma-Aldrich, Germany. RPMI-1640 medium and supplements for cell proliferation, as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components were purchased from Sigma-Aldrich. Distilled water was used for all polymerizations and preparations of buffer solution.
Babić Radić M.M., Filipović V.V., Vukomanović M., Nikodinović Runić J, & Tomić S.L. (2021). Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials. Gels, 8(1), 22.
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4

Hydrogel Biomaterials for Bioactive Compound Delivery

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2-Hydroxyethyl methacrylate (H, 99%) monomer and polymers of natural origin—gelatin from porcine skin (G, gel strength 300, Type A) and alginate (A)—were supplied from Sigma-Aldrich, Burghausen, Germany. Cross-linking agents N-hydroxysuccinimide (NHS) and N-ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and polymerization agensts potassium persulfate (PPS) and N,N,N′,N′-tetramethylene diamine (TEMED) were purchased from Sigma-Aldrich, St. Louis, MO, USA. RPMI-1640 medium and supplements for cell proliferation as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components were purchased from Sigma-Aldrich, St. Louis, MO, USA. All polymerizations and buffer preparations were performed in deionized water. Resveratrol (RSV) as a bioactive agent was purchased from Sigma-Aldrich, Germany.
Vuković J.S., Filipović V.V., Babić Radić M.M., Vukomanović M., Milivojevic D., Ilic-Tomic T., Nikodinovic-Runic J, & Tomić S.L. (2022). In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds. Polymers, 14(20), 4459.
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