Nicolet 6700 ftir spectrophotometer

The FT-IR spectra were collected with a Nicolet 6700 FT-IR spectrophotometer (Thermo Scientific, Waltham, MA, USA) equipped with a single reflection diamond ATR cell. Samples were deposited at the diamond surface by evaporation under a nitrogen stream. Infrared absorption spectra were recorded in the region between 4000 and 400 cm⁻¹ with a resolution of 4 cm⁻¹. FT-IR spectra were analyzed with an application of ORIGIN software (v.9.0 PRO, OriginLab Corporation, Northampton, MA, USA). To determine changes in the secondary structure of gliadins, difference spectra in the amide I region were calculated. The spectrum of the control sample (gliadins) was subtracted from spectra of samples containing gliadins and phenolic acids.

Dithizone, mercuric chloride (HgCl₂), triethylamine (Et₃N), 2-nitroaniline, 3-nitroaniline and 4-nitroaniline were purchased from Aldrich. DNA (deoxyribonucleic acid) was purchased from Acros Organics. Solvents such as chloroform, DMF and ethanol were purchased from Aldrich, and petroleum ether and ethyl acetate were purchased from Fischer Scientifics; all these solvents were purified before use according to the reported protocol [13 ]. Melting points were measured on BIO COTE Model SMP10 melting apparatus. Elemental composition was estimated on Thermo Scientific Flash 2000 organic elemental analyser. FTIR was recorded on Thermo Scientific Nicolet-6700 FTIR spectrophotometer. NMR spectrum was recorded on BRUKER AVANCE 300 MHz NMR spectrometer and UV-visible spectrum was recorded on UV-visible spectrophotometer Shimadzu 1800.

UV-Vis absorption spectrum was acquired by utilizing a spectrophotometer of TU-1901 UV-Vis (Beijing Puxi Inc., China). With a Cary Esclipse spectrofluorimeter (Varian, United States), photoluminescence (PL) experients were performed. X-ray photoelectron spectroscopy (XPS) with Mg Kα excitation (1,253.6 eV) was gathered in a VG ESCALAB MKII spectrometer (VG, Britain). Through a X-ray diffractometer system of Bede D1 high-resolution(Bede Co., United Kingdom), X-ray diffraction (XRD) spectra were recorded. Making use of a Nicolet 6700 FTIR spectrophotometer (Thermo Fisher Scientific, United States)., Fourier transform infrared (FTIR) spectroscopy was estimated at wave numbers ranging from 500 cm⁻¹ to 4,000 cm⁻¹. operating a JEM-2100 transmission electron microscope (TEM) at 200 kV (JEOL, Japan), resultant CDs' morphology and mean diameter were characterized. All the experiments and measurements were carried out at the room temperature under the ambient conditions.

Thermal behavior of the investigated compound was determined using Setaram Setsys (Setaram Instrumentation, Ratingen, Germany)16/18 derivatograph, registering TG, DTG and DSC curves. The sample (3.96 mg) was heated in a Al₂O₃ crucible between 30 and 1000 °C in flowing air atmosphere (v = 0.75 dm³ h⁻¹) with a heating rate of 10 °C min⁻¹. The temperature and heat flow of the instrument were calibrated by the melting point and enthalpy of indium standard. TG-infrared spectrometry (TG-FTIR) of the title compound was performed using the TGA Q5000 analyzer (TA Instruments, New Castle, DE, USA) interfaced to the Nicolet 6700 FTIR spectrophotometer (Thermo Scientific, Waltham, MA, USA) in oxidizing and inert atmospheres. The samples of 10.65 mg (air atm.) and 19.09 mg (N₂ atm.) were put in an open platinum crucible and heated from an ambient temperature (~25 °C) to 700 °C with a heating rate of 20 °C min⁻¹ (at a flow rate of 25 mL min⁻¹). In order to reduce the possibility of gases condensing along the transfer line, the temperature in the gas cell and transfer line were set to 250 and 240 °C, respectively. The FTIR spectra were recorded in the spectral range of 600–4000 cm⁻¹ with a resolution of 4 cm⁻¹ and 6 scans per spectrum. Thermal decomposition of compound in air atmosphere using TGA Q5000 analyzer was also performed with a heating rate of 10 °C min⁻¹ in the range 25–700 °C.

FT-IR spectra were recorded using a Thermo Scientific Nicolet 6700 FT-IR spectrophotometer. A thin layer of sample was placed in direct contact with an infrared attenuated total reflection (ATR) diamond crystal. All FT-IR spectra were collected in the wavenumber range of 4000 to 500 cm⁻¹ by co-adding 32 scans with a resolution of 4 cm⁻¹. Differential scanning calorimetry (DSC) was performed on a Mettler Toledo Star DSC system. Nitrogen was used as a purge gas (10 ml·min⁻¹). Samples between 2 and 5 mg were placed in aluminum pans and heated from 25 to 450°C with a heating rate of 20°C/min. Thermogravimetric analysis (TGA) was recorded using a Mettler Toledo Star TGA/DSC unit. Nanoparticles (1–2 mg) were placed in the aluminum pans (40 μl) and heated from 50 to 800°C with a heating rate of 10°C/min. The morphology of MNPs was studied with transmission electron microscopy TEM/EDX (Tecnai G2 X-TWIN). A drop of the nanoparticle dispersion in THF was deposited onto a carbon-coated copper grid and dried in a vacuum oven at temperature 50°C.