Larvae were positioned in the center of a 35-mm dish filled with 10% MMR. The temperature of the dish was regulated during testing with a bipolar temperature controller (CL-100) and quick exchanged heated/cooled platform (QE-1HC, Warner Instruments). Larvae were tested at either cold or warm temperature (14.5 or 22.5 ± 0.5°C, respectively). A hand-held mounted eyebrow hair was used to lightly stroke the larva from the back of the head, along the side until the mid-tail of the resting larva, or until the larva swam away. Responses were video recorded at 30 Hz. Each larva was tested at least 20 times with at least 5 s rest period between trials. Six larvae from at least two independent sibling cohorts were tested per treatment group. Stage 37/38 and 40 larva responses were evaluated by an experimenter blind to the temperature treatment, and categorized by swimming away (swim), flexing to either side (flexion), or no response as described by Roberts and colleagues [26 , 27 (link)]. Swimming responses for stage 40 larvae were traced and analyzed using NIS Elements software (Nikon).
Qe 1hc
Manufactured by Warner Instruments
The QE-1HC is a high-capacity peristaltic pump designed for laboratory and industrial applications. It features a variable speed control and can accommodate a wide range of flow rates. The pump is constructed with durable materials to provide reliable and consistent performance.
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Lab products found in correlation
2 protocols using qe 1hc
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Larval Tactile Response Assay
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Larval Tactile Response Assay
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Larvae were positioned in the center of a 35-mm dish filled with 10% MMR. The temperature of the dish was regulated during testing with a bipolar temperature controller (CL-100) and quick exchanged heated/cooled platform (QE-1HC, Warner Instruments). Larvae were tested at either cold or warm temperature (14.5 or 22.5 ± 0.5°C, respectively). A hand-held mounted eyebrow hair was used to lightly stroke the larva from the back of the head, along the side until the mid-tail of the resting larva, or until the larva swam away. Responses were video recorded at 30 Hz. Each larva was tested at least 20 times with at least 5 s rest period between trials. Six larvae from at least two independent sibling cohorts were tested per treatment group. Stage 37/38 and 40 larva responses were evaluated by an experimenter blind to the temperature treatment, and categorized by swimming away (swim), flexing to either side (flexion), or no response as described by Roberts and colleagues [26 , 27 (link)]. Swimming responses for stage 40 larvae were traced and analyzed using NIS Elements software (Nikon).
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