D 10 analyzer

Manufactured by Bio-Rad

Sourced in United States

The D-10 analyzer is a laboratory instrument designed for the analysis of hemoglobin variants and glycated hemoglobin (HbA1c) levels. It utilizes high-performance liquid chromatography (HPLC) technology to separate and quantify different hemoglobin fractions in a patient's blood sample.

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Lab products found in correlation

7600 020 autonomic analyzer, by Hitachi (2 mentions) Unicel dxc 800 analyzer, by Beckman Coulter (2 mentions) 7600 020 chemical analyzer, by Hitachi (1 mentions) Centaur xp immunoassay system, by Siemens (1 mentions) Xn 10 analyzer, by Sysmex (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Cobas mira s analyzer, by Roche (1 mentions) Cobas e411, by Roche (1 mentions) Synchron sh9 pro, by Beckman Coulter (1 mentions) Immulite 2000, by Siemens (1 mentions) Cobas integra analyzer, by Roche (1 mentions) Advia 1800 analyzer, by Siemens (1 mentions) Accu chek aviva, by Roche (1 mentions) Hem 7120, by Omron (1 mentions) 7180 automatic biochemical analyzer, by Hitachi (1 mentions) 7180 automatic analyzer, by Hitachi (1 mentions) Contour next, by Bayer (1 mentions)

19 protocols using d 10 analyzer

Metabolic Biomarkers in Fasting and Postprandial States

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All the measurements were made after a 10-h overnight fast. Waist circumference, hip circumference, and blood pressure were measured while participants were barefoot and wearing light clothes after defecation. Blood samples were collected before and 2 h after a fixed breakfast (~300 kcal) for measurement of hemoglobin A1c (HbA1c), fasting lipid profile, fasting and 2-h postprandial plasma glucose (2hPPG), insulin, and C-peptide. Fasting and postprandial plasma glucose levels, lipid concentrations for total cholesterol, triglycerides, and HDL-cholesterol (HDL-c) were measured using an automated enzymatic method (7600-020 Chemical Analyzer, Hitachi, Japan). HbA1c levels were determined by high-performance liquid chromatography (D-10 analyzer, Bio-Rad, Hercules, CA). Fasting and 2-h postprandial plasma insulin and C-peptide were measured by radioimmunoassay (Centaur XP immunoassay system, Siemens Healthcare Diagnostics, New York, NY).
Homoeostasis model assessment (HOMA) was used to estimate basal β-cell function (HOMA-B) and insulin resistance (HOMA-IR). HOMA-B was calculated as [20× fasting insulin (FINS)] / [fasting plasma glucose (FPG)—3.5]. HOMA-IR was calculated as FPG × FINS / 22.5 [14 (link)].

Yang X., Xu W., Zhu Y., Deng H., Tan Y., Zeng L, & Weng J. (2018). Decreased β-Cell Function is Associated with Cardiovascular Autonomic Neuropathy in Chinese Patients Newly Diagnosed with Type 2 Diabetes. Neuroscience Bulletin, 35(1), 25-33.

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Standardized Biomarker Assessment Protocol

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The measurements of height and weight, based on a standardized form, were recorded by the same physician. Body mass index (BMI) was calculated as body weight (kg) divided by the square of the height (m). The blood pressure level was calculated by the mean of two consecutive blood pressure values 10 minutes apart taken in a sitting position. Blood and urine samples were collected after a 10-hour overnight fast. Serum concentrations of total cholesterol (TC), triglycerides (TG), LDL-cholesterol (LDL-c), HDL-cholesterol (HDL-c), fasting plasma glucose, and creatinine (Cr) were obtained via an automated enzymatic method (Hitachi, Japan, 7600-020 autonomic analyzer), and high-pressure liquid chromatography (Bio-Rad, USA, D-10 analyzer) was used to evaluate the HbA1c level. The eGFR was estimated by the simplified MDRD equation [16 (link)]. The concentration of serum CysC was measured by particle-enhanced turbidimetric immunoassay (Hitachi, Japan, 7600-020 autonomic analyzer).

Yang X., Lin Q., Li X., Wu L., Xu W., Zhu Y., Deng H., Zhang Y, & Yao B. (2019). Cystatin C Is an Important Biomarker for Cardiovascular Autonomic Dysfunction in Chinese Type 2 Diabetic Patients. Journal of Diabetes Research, 2019, 1706964.

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Comprehensive Clinical Data and Metabolic Profiles

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General clinical data of the subjects were collected, including gender, age, smoking status, duration of T2DM, body mass index (BMI), and Wagner DFU grade.⁶ Fasting venous blood was obtained, and blood routine related parameters including white blood cell count (WBC), platelet count (PLT), and lymphocyte count (LYM) were measured using a Sysmex XN‐10 analyzer (Sysmex). The PLR was calculated as the platelet‐to‐lymphocyte ratio. Triglyceride (TG), total cholesterol (TC), high‐density lipoprotein (HDL), low‐density lipoprotein (LDL), and fasting blood glucose (FPG) were measured using an Olympus AU5421 analyzer (Olympus), and glycated hemoglobin (HbA1c) was measured using a Bio‐Rad D10 analyzer (Bio‐Rad).

Zhang K., Ding S., Lyu X., Tan Q, & Wang Z. (2021). Correlation between the platelet‐to‐lymphocyte ratio and diabetic foot ulcer in patients with type 2 diabetes mellitus. Journal of Clinical Laboratory Analysis, 35(4), e23719.

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Induction of Diabetes in Mice

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CWRU IACUC and LSVAMC ACORP approved the animal protocols employed in this study. C57BL/6J and IL-17A^−/− mice were obtained from Jackson Laboratories. Diabetes was induced in 8–10 week old male mice by intraperitoneal injections of streptozotocin (60 mg/kg) on 5 consecutive days, as previously described^{7 (link)–9 (link)}. Alternatively, heterozygous and homozygous B6.BKS(D)-Lepr^dblJ mice (db/db) were examined at 8–10 weeks of age, during spontaneous diabetic onset for systemic IL-17A analysis. Diabetes was defined by non-fasted blood glucose concentrations greater than 250 mg/dl, which was verified using glucose-dehydrogenase-based strips on three consecutive occasions beginning 1 week after the last STZ injection; hyperglycemia was also quantified by hemoglobin A1c levels using a Bio Rad D-10 analyzer. Insulin (0–0.2 U) was administered as needed to maintain proper body weight.

Lindstrom S.I., Sigurdardottir S., Zapadka T.E., Tang J., Liu H., Taylor B.E., Smith D.G., Lee C.A., DeAngelis J., Kern T.S, & Taylor P.R. (2019). Diabetes induces IL-17A-Act1-FADD-dependent retinal endothelial cell death and capillary degeneration. Journal of diabetes and its complications, 33(9), 668-674.

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Biochemical Analyses in Clinical Research

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Lipid parameters were analyzed in the Lipid Laboratory of the Institute for Clinical and Experimental Medicine, Prague, Czech Republic, employing a fully automated enzymatic method (COBAS MIRA S analyzer, Roche Diagnostics, Basel, Switzerland), along with enzymatic kits produced by the same manufacturer. LDL-cholesterol was calculated using the Friedewald formula. Serum and urine creatinine were analyzed using an enzymatic assay (Hitachi 902 autoanalyzer, Roche Diagnostics, Basel, Switzerland). Glycosylated hemoglobin (HbA1c) was assessed using HLCP method (D-10 analyzer, Bio-Rad, Hercules, California, USA), whereas urine albumin was determined using an immunoturbidimetry (COBAS MIRA S analyzer, Roche Diagnostics, Basel, Switzerland); both these analyses were performed by the Department of Clinical Biochemistry at the Thomayer University Hospital, Prague, Czech Republic. The accuracy of these analyses was continuously monitored and tested, with external quality control of lipid parameters performed by the Centers for Disease Control and Prevention (Atlanta, Georgia, USA).

Cífková R., Harazny J.M., Bruthans J., Wohlfahrt P., Krajčoviechová A.H., Lánská V., Gelžinský J., Mateřánková M., Mareš Š., Filipovský J., Mayer O J.r, & Schmieder R.E. (2023). Early vascular damage in retinal microcirculation in arterial hypertension: the Czech post-MONICA study. Journal of Hypertension, 42(3), 557-563.

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Plasma Glucose and HbA1c Measurement Techniques

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Plasma glucose concentration was measured with glucose oxidase method on Beckman Coulter Unicel DXC-800 analyzer. HbA1c was measured using ion-exchange high-performance liquid chromatography (Bio-Rad D-10 analyzer). The reference range of the test was from 3.8% to 18.5%.
Contour^® Next glucometer was used for CBG measurements (manufactured by Bayer HealthCare LLC). Fingertips were used for CBG sample using standard lancing device provided by the manufacturer. The test strips used flavin adenine dinucleotide-glucose dehydrogenase method for blood glucose testing.[8 (link)] The results of the glucometer are referenced to plasma/serum blood glucose values. The measuring range of the glucometer is 10–600 mg/dl.

Lakhani O.J., Kumar S., Tripathi S., Desai M, & Seth C. (2017). Comparison of Two Protocols in the Management of Glucocorticoid-induced Hyperglycemia among Hospitalized Patients. Indian Journal of Endocrinology and Metabolism, 21(6), 836-844.

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Evaluating Laboratory Markers in T1D

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Laboratory parameters were measured in blood samples in the Department of Laboratory Medicine of the National Institute of Children’s Diseases in Bratislava.
Glucose, pH, and bicarbonate levels were determined using standard laboratory methods. HbA1c was evaluated from whole blood using an HPLC method (D-10 analyzer, Bio-Rad, Hercules, CA, USA). Fasting serum C-peptide levels were determined using the electrochemiluminescence immunoassay method (Cobas e411, Roche Diagnostic, Basel, Switzerland). Pancreatic autoantibodies (against glutamic acid decarboxylase, tyrosine phosphatase, and insulin were detected using commercial ELISA kits (RSR Ltd., Cardiff, UK), according to the manufacturer’s instructions. Mean HbA1c was calculated using all obtained values during the first year of the follow-up. Delta HbA1c was calculated as a difference in HbA1c values at the time of T1D diagnosis and after one year of T1D duration. HbA1c values were transformed to the International Federation of Clinical Chemistry values (http://www.ngsp.org/ifccngsp.asp, accessed on 7 June 2008) and Diabetes Control and Complications Trial values.

Podolakova K., Barak L., Jancova E., Stanik J., Sebekova K, & Podracka L. (2022). The Bright Side of Skin Autofluorescence Determination in Children and Adolescents with Newly Diagnosed Type 1 Diabetes Mellitus: A Potential Predictor of Remission?. International Journal of Environmental Research and Public Health, 19(19), 11950.

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Evaluating Glycemic Control through HbA1c and eAG

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The random blood glucose and HbA1c levels of 461 patient samples (178 male and 283 female) were included in the study. Blood samples were taken on the same day for the determination of both RBS and HbA1c. The eAG levels (mg/dL) were calculated using the following formula: 28.7 × HbA1c – 46.7. The samples were divided into four groups on the basis of HbA1c levels as group 1: HbA1c greater than 9% (poorly controlled diabetic), group 2: HbA1c 6.5 to 9% (fairly controlled diabetic), group 3: HbA1c 5.7 to 6.4% (prediabetic), and group 4: HbA1c less than 5.7% (nondiabetic).
8 (link)
Glucose levels were determined using the glucose oxidase method in Transasia Erba analyzer with commercially available Agappe kits. HbA1c levels were determined using high-performance liquid chromatographic method on Biorad D10 analyzer with Biorad kits.

Garg P., Pethusamy K, & Ranjan R. (2022). Correlation between Estimated Average Glucose Levels Calculated from HbA1c Values and Random Blood Glucose Levels in a Cohort of Subjects. Journal of Laboratory Physicians, 15(2), 217-223.

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Biochemical and Lipid Profile Analysis

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Serum samples were analyzed for urea, albumin, total protein, total cholesterol (TC), high density lipoprotein (HDL) cholesterol, triglycerides (TG), uric acid, alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine transaminase (ALT) using Human commercial kits and Humalyzer 3500. Moreover, glycated hemoglobin (HbA1c) test was performed to find out the amount of glycated hemoglobin (HbA1c) using Bio Rad D-10 analyzer. Very low density lipoprotein (VLDL) cholesterol and Low density lipoprotein (LDL) cholesterol concentrations were calculated using the following Friedewald equations [12] (link):

The atherogenic indices were calculated as follows:

[13] (link)

[14] (link)

[15] (link)

[16] (link)

Uddin N., Hasan M.R., Hasan M.M., Hossain M.M., Alam M.R., Hasan M.R., Islam A.F., Rahman T, & Rana M.S. (2014). Assessment of Toxic Effects of the Methanol Extract of Citrus macroptera Montr. Fruit via Biochemical and Hematological Evaluation in Female Sprague-Dawley Rats. PLoS ONE, 9(11), e111101.

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Comprehensive Metabolic and Renal Assessment

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HbA1c in erythrocytes was measured by high-performance liquid ion exchange chromatography (D-10 analyzer (Bio-Rad, Hercules, CA, USA). The glucose oxidase method was used for measuring capillary glucose. The average daily hyperglycemia was determined.
The serum lipid content (TG, TC, and HDL) was determined using commercial kits (BioSystems, Spain) and a biochemical analyzer (Synchron SH9 Pro, Beckman Coulter, Brea, CA, USA). The levels of LDL were calculated using the Friedewald formula [34 (link)]: LDL = TC − (HDL + VLDL)). The level of VLDL equaled TG/2.2.
Methods used for kidney damage determination in the early studies included a GFR calculation, urinary albumin and ratio of albumin/creatinine. These were determined on a biochemical analyzer (Synchron SH9 Pro, Beckman Coulter, Brea, CA, USA) using the immunoturbidimetric method. The Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula was used for calculating GFR (mL/min/1.73 m²).

Darenskaya M., Chugunova E., Kolesnikov S., Semenova N., Michalevich I., Nikitina O., Lesnaya A, & Kolesnikova L. (2022). Receiver Operator Characteristic (ROC) Analysis of Lipids, Proteins, DNA Oxidative Damage, and Antioxidant Defense in Plasma and Erythrocytes of Young Reproductive-Age Men with Early Stages of Type 1 Diabetes Mellitus (T1DM) Nephropathy in the Irkutsk Region, Russia. Metabolites, 12(12), 1282.

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