0.2 μmnylon filters

Manufactured by Merck Group

Sourced in United States

The 0.2 μm nylon filters are laboratory equipment designed for filtration applications. They are made of nylon and have a pore size of 0.2 micrometers. These filters are used to remove particles, microorganisms, and other contaminants from various liquids or gases during laboratory processes.

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Lab products found in correlation

Tartaric, by Merck Group (1 mentions) Malic, by Merck Group (1 mentions) Oxalic, by Merck Group (1 mentions) Lactic, by Merck Group (1 mentions) Turbochrom software, by PerkinElmer (1 mentions) Ascorbic acid, by Merck Group (1 mentions)

2 protocols using 0.2 μmnylon filters

Cellulose Carbanilation for GPC Analysis

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Cellulose
was prepared for GPC analysis by modification by phenyl isocyanate
to form cellulose carbanilates.^{39 (link)} A volume
of 100 μL of phenyl isocyanate in 500 μL of pyridine was
added to 1 mg of cellulose under a stream of dry argon gas. Samples
were sealed in ampoules and rotated slowly at 70 °C for 48 h.
Unreacted phenyl isocyanate was quenched with 1.2 mL of methanol.
Samples were dried overnight at 40 °C under a stream of dry argon
gas, dissolved in tetrahydrofuran, and filtered through 0.2 μm
nylon filters (Millipore). GPC was performed with a KD-806M column
(Shodex) using tetrahydrofuran. A specific refractive index (dn/dc) of 0.169 mL g^–1^{4 (link)} was used for cellulose tricarbanilates
in tetrahydrofuran. Enzymatic synthesis of cellulose from UDP-Glc
was performed with UDP-[¹⁴C]-Glc, as described by McManus
et al.^{11 (link)} All reactions were performed at
30 °C or as specified in the figure legends. The synthesized
cellulose was treated with 2% sodium dodecyl sulfate (SDS) and then
solubilized with 8% LiCl (w/v) in dimethylacetamide for GPC analysis,
as described previously.^{25 (link)} Radioactivity
was quantified by a Beckman Coulter LS6500 scintillation counter using
ScintiVerse (Fisher Scientific) scintillation fluid.

McManus J.B., Yang H., Wilson L., Kubicki J.D, & Tien M. (2018). Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis. ACS Omega, 3(3), 2690-2698.

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Phosphate Solubilization Kinetics of Strain KPS-11

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The strain KPS-11 was inoculated in 100 mL of Pikovskaya’s broth in 500 mL flasks in triplicate and incubated in an orbital shaker at 150 rpm at 28 ± 2°C for up to 10 days (240 h). Twenty micro liter of bacterial culture from each flask was harvested at 5, 7, and 10 days post inoculation, centrifuged at 13,000 × g for 10 min and cell-free supernatant was collected. Phosphate solubilization was determined through Phospho-molybdate blue color method using spectrophotometer (λ = 882 nm) as described by Murphy and Riley (1962) (link). For HPLC analysis, the cell-free supernatant was filtered through 0.2 μm nylon filters (Millipore, USA) and 20 μL was injected to HPLC equipped with Turbochrom software (Perkin Elmer, USA) and C-18 column at a flow rate of 0.6 mL min^-1 using 30:1:70 (v/v/v) methanol: acetic acid: water as mobile phase. Signals were detected at 210 nm. The organic acids gluconic, malic, lactic, oxalic, tartaric, and ascorbic acid (Sigma–Aldrich) were used as standard.

Hanif M.K., Hameed S., Imran A., Naqqash T., Shahid M, & Van Elsas J.D. (2015). Isolation and characterization of a β-propeller gene containing phosphobacterium Bacillus subtilis strain KPS-11 for growth promotion of potato (Solanum tuberosum L.). Frontiers in Microbiology, 6, 583.

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