P erk thr202 thr204

Manufactured by Cell Signaling Technology

The P-Erk Thr202/Thr204 is a laboratory reagent used to detect and quantify the phosphorylation of extracellular signal-regulated kinase (Erk) at specific threonine residues (Thr202 and Thr204). It is commonly used in cellular and molecular biology research to study signal transduction pathways and cell signaling events.

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P-ERK/ERK P-ERK

2 protocols using p erk thr202 thr204

Antibody-based Kinase Signaling Analysis

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Antibodies were obtained from commercial sources. Antibodies against Aurora kinase A (#12100), p-Aurora A Thr288/Aurora B Thr 232 (#2914), EGF receptor (EGFR) (#2232), p-EGFR Tyr1068 (#3777), p-GSK-3β Ser9 (#9336), p-Akt Ser473 (#4060), Akt (#4691), p-Erk Thr202/Thr204 (#4370), Erk 1/2 (#9102), poly(ADP-ribose) polymerase (PARP) (#9542), PKC-α (#2056), PKC-δ (#2058), PKC-ε (#2683), and p-Serine PKC substrates (#6967) were purchased from Cell Signaling Technology (Beverly, Massachusetts). Antibody against Aurora B (#ab45145) was purchased from Abcam (Cambridge, UK). Antibody against GSK-3β (#610201) was purchased from BD Bioscience (San Jose, California). Antibodies against Histone H3 (#sc-10809), PKC-βII (#sc-210) and γ-tubulin (#sc-51715) were purchased from Santa Cruz Biotechnology (Santa Cruz, California). Antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#2275-pc-100) was purchased from Trevigen (Gaithersburg, Maryland). The antibody against p-Histone H3 Ser10 (#06-570) was purchased from Millipore (Billerica, Massachusetts). Horseradish peroxidase-conjugated secondary antibodies against rabbit and mouse IgG were purchased from Dako (Glostrup, Denmark).

Kawai M., Nakashima A., Kamada S, & Kikkawa U. (2015). Midostaurin preferentially attenuates proliferation of triple-negative breast cancer cell lines through inhibition of Aurora kinase family. Journal of Biomedical Science, 22(1), 48.

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Quantitative Western Blot Analysis

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Western blot was performed as described previously (31 (link)). Briefly, equal protein samples were loaded into a 4–20% HCL gel, transferred to a nitrocellulose membrane, and incubated overnight in 1:500–1:1,000 antibody dilutions in 5% milk with Tris-buffered saline with Tween for phosphorylated (p)AT^ser473, total AKT, pFOXO1^ser256, total FOXO1, pERK^{thr202/thr204} (Cell Signaling Technology), pIR^tyr972 (Invitrogen), and total ERK (Santa Cruz). Image J software was used to quantify mean intensity of equal-area sections representing each sample.

Landry T., Laing B.T., Li P., Bunner W., Rao Z., Prete A., Sylvestri J, & Huang H. (2020). Central α-Klotho Suppresses NPY/AgRP Neuron Activity and Regulates Metabolism in Mice. Diabetes, 69(7), 1368-1381.

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